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Questions related from Lesly Castillo
Hello, I am currently attempting to obtain amplifications of SoxC from a species of onychophoran belonging to the Peripatidae family using primers designed from a sequence of its sister family,...
31 March 2024 1,408 4 View
What would be the usefulness of quantifying cDNA before conducting a PCR, and how could it influence the effectiveness and reproducibility of the obtained results?
21 March 2024 637 3 View
21 March 2024 4,595 4 View
In the gel that I made with the same cDNA but with different sets of primers, I had the results that are seen in the image in the 6 wells before the marker, it is the same primer starting with the...
16 September 2023 2,029 0 View
Hello, I've been having issues with my cDNA PCR which used to work previously. I'm working with cDNA from velvet worms, amplifying Cox1. Earlier, I had successfully amplified Cox1 with specific...
23 August 2023 6,232 4 View
What concentrations of reagents are recommended to start standardizing an RT-PCR primer set?
23 August 2023 8,312 3 View
I am doing a gradient PCR to achieve standardization of my primer set, however the results I have obtained from it are inconclusive. What do you recommend me to do? The image shows tm tests from...
18 May 2023 1,987 5 View
We want to perform a gradient PCR for our primers whose suggested Tm's are between 49.6°C and 50.7°C. We want to test with temperatures ranging from 47 to 55°C. Is this setup correct?
12 May 2023 734 3 View
why in my gel the loading dye is not distributing evenly? the loading dye is SafeView™ Classic
20 April 2023 9,733 1 View
