Hello everybody,
Concerning the coupling reaction via amide bond formation between an carboxylic acid (CA) bearing metal complex and low molecular weight chitosan, which conditions would you prefer? As the complex itself is very good water soluble I was thinking of CA activation in water using EDC/NHS. I read a lot about buffer needed within this reaction due to pH dependency, but is this really necessary when I activate my CA and subsequently add my chitosan? And talking about adding, is it working when I add just the chitosan powder hoping that the product gets soluble? Or do I have to dissolve the polymer first in the usual 1% acetic acid?
Every answer and ideas will be highly appreciated!
Have a good one!
Dear Michael,
that reference IS a start indeed! Thanks a lot for your quick answer.
Have a lovely week.
Michael
This is Polyethylene Glycol
Used in Highly Controlled condition
Use in PPM level with pH controll.
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