Alternative PCR array software?

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How to interpret this sentence making a HPLC mobile phase?

I'm following a paper for their HPLC methodology, but I'm having a hard time understanding how to make mobile phase A. To quote: "Mobile phase A is a mixture containing 0.2% potassium dihydrogen...

13 June 2024 8,321 1 View

What is the reason for certain size measurement errors using Dynamic Light Scattering?

I am currently doing some initial tests on the size of carboxylate-modified polystyrene beads in deionised water to determine their interaction with BSA with and without the addition of PLL-g-PEG...

09 June 2024 5,603 7 View

Marker Features variance by cluster, sample, and treatment group?

Hello! I am comparing scATAC-seq gene markers in ArchR using Seurat's getMarkerFeatures function. After applying Log2FC and FDR thresholds, I get different output depending on if I groupBy...

09 May 2024 1,252 0 View

How can laboratories improve antibiotic selections for uncommon but potentially fatal pathogens?

Providers should make an effort to understand local resistance patterns including those of the referring hospitals. Such data are relevant to monitor longitudinal resistance trends and formulate...

18 February 2024 933 1 View

How can the precision and accuracy of the Sysmex DI-60 be enhanced, specifically in recognizing and classifying abnormal red blood cell morphology?

Sysmex DI-60 was found to be dependable in the characterization of red blood cells (RBCs). However, in the case of schistocytes, the study revealed high sensitivity with poor specificity, which...

06 February 2024 3,484 1 View

Comsol RF MODULE ?

hi, I would like to know if it is possible to provide voltage excitations for primary and secondary coils in Comsol Multi-physics using the RF module instead of the AC/DC module. I would like to...

03 January 2024 6,433 0 View

What is the germination protocol of navara paddy seeds?

Navara rice which is the traditional rice from kerala

27 December 2023 7,544 1 View

Why do i have an decreasing number of pores in R10.4.1 flow cell (nanopore)?

The number of pores in the R.10.4.1 flow cell decreased significantly from +/- 1400 to 291 after nanopore sequencing with only 24 samples multiplexing. I used the SQK-RPB114-24 kit for processing...

18 December 2023 9,180 0 View

Why does my protein precipitate on Ni-NTA column during purification?

Hello all, I am currently trying to purify a fragment of the large surface protein of HBV however during purification I notice that a white precipitate form on the Ni-NTA column. (At pH 8.0; pI of...

07 November 2023 7,131 4 View

Are you aware of available data of the Ross Ice Shelf front during 2022?

Hi everyone, I wanted to ask if anyone of you is aware of a potential source/dataset to derive the ice shelf front/coastline of the Ross Ice Shelf during 2022? There are some great datasets...

26 September 2023 1,420 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Someone have the key or the installer of the program Mx pro 3000 (PCR real time)?

I need to install this program

25 July 2024 4,756 0 View

Illustra™ MicroSpin™ G-25 columns what it is used for?

Hello, We found three packages of Illustra™ MicroSpin™ G-25 columns in the cabinet of an unused lab. They are very old but have never been opened. I have never used this kit before, and I couldn't...

25 July 2024 4,927 3 View