Naomi Hitefield

3 Questions 6 Answers 0 Followers

Questions related from Naomi Hitefield

Mitochondrial Membrane Uncoupler Suggestions in 96-Well Assay?

We have a 96-well assay we use to measure mitochondrial membrane potential. A 200 uL volume of cell resuspension is in each well. We want to add a membrane uncoupler, like FCCP, to uncouple the...

16 January 2019 7,092 0 View

Has anyone received negative RFU values for one of your replicates in qPCR?

We are running qPCR with SYBRGreen to amplify an inserted 12S ribosomal DNA region of a plasmid. When we ran this amplification for the first time (in duplicate), one replicate looked fine while...

04 April 2018 5,043 4 View

Can you help with TaqMan SNP Genotyping Assay analysis?

Hi! We've recently purchased Fisher TaqMan SNP APOE Genotyping Assays and have been running them on brain samples with the BioRad CFX96 thermocycler. Test brain samples are supposed to be...

16 January 2018 2,926 11 View