Which is the best mitochondrial potential dye for primary cells?

Hi! I am wondering if someone can recommend me a good dye for measuring mitochondrial potential. I have used TMRE with good results in the past, but I have heard also about Mitoview. I would use...

12 January 2021 4,200 4 View

How to analyze sodium lauryl sulfate using HPLC-RID?

Hi, I am analyzing the content of anionic surfactant: sodium lauryl sulfate (SLS) in commercial detergents using HPLC-RID/refractive index detector. I used column Agilent Zorbax Eclipse XDB C-18...

15 November 2020 1,405 2 View

How do I test for gender differences in Spearman rho correlations?

I want to explore if there is a gender difference in a correlation between two ordinal items. I have calculated spearman rho within gender for the items, but I now need to test if the correlation...

09 October 2020 970 13 View

How to get rid of Matrigel during enteroid passaging?

Hello. I am trying to generate monolayers from cow enteroids. The protocol I am following involves 0.5uM EDTA and Trypsin-EDTA 0.05%, each enzyme is applied in two different steps. But for some...

10 March 2020 1,805 1 View

Is there a way to edit the citations in MENDELEY so it's in the order of citation in the text body instead of Author?

Does anyone know how to change the citation style in Mendely in order to sort the references according to the order of citation in the paper? So that in the text is displayed only the number (eg....

01 January 2020 746 2 View

How to avoid impurities in immunoprecipitation with magnetic beads?

Hello, I need to extract an antigen from human serum. I tried with streptavidine-coated magnetic beads and a pair of biotinylated antibodies. Then I followed the elution procedure, as recommended...

09 September 2019 6,947 4 View

How to analyze alkyl distribution of Methyl Ester Sulfonate / Sulfonated Methyl Ester?

I need to analyze the alkyl distribution of Methyl Ester Sulfonate to know the molecular weight of my sample. I tried to use gas chromatography to do this. My method was to reflux the sample first...

14 July 2019 2,133 4 View

Can you recommend me some articles that dealing with the electronic transitions of toluene?

Hello! I'm working on assigning absorption bands to UV-vis spectra of unknown matrix. I think some are due to the presence of toluene, but I can't find any article that describes the electronic...

19 March 2019 8,119 3 View

What are these spheres created by genetically modified Ralstonia eutropha after fermentation of hemp?

Hello microbiology researchers working with Ralstonia eutropha. The organism we have created contains two genes that allow R. eutropha to ferment hemp and produce P4HB. When we began the culture...

13 November 2018 3,838 3 View

Which are the incubation conditions for a biotinylated antibody with a streptavidin coating?

I have to do a sandwich coating with: 1) biotinylated BSA 2) streptavidine 3) biotinylated antibody Which are the common conditions for the incubation regarding each step? Buffer type and pH,...

08 November 2018 770 2 View

How to inter pain intensity data using NRS when patients report their pain in spectrum?

I have been working on a pain intensity study and I wanted my patients to report their pain intensity using numerical rating scale. many patients have reported thier pain in spectrum like 2 to 3...

13 February 2021 6,598 3 View

What statistical test is required for an analysis on pre-COVID & post-COVID patient improvement after attending a 10-week programme?

Hi all, Myself and my supervisor are looking at carrying out a statistical analysis to see whether there is a difference in patient outcomes for our 10-week pain management programme according to...

10 February 2021 6,622 10 View

Mental health of COVID-19 Patients?

We want to conduct a study on the mental health and psychological problems in COVID-19 patients and would measure it in terms of Anxiety by Beck Anxiety Inventory, Depression by Patient Health...

02 February 2021 6,401 6 View

I.P. drug injection- time to reach brain?

I am injecting different serotonergic drugs into mice i.p. and see an effect in the brain electrophysiology quite fast, i.e. within minutes, when using a specific receptor agonist drug. I do not...

01 February 2021 8,406 3 View

I'm looking for a dataset of eeg recorded during the pain stimulation. Anyone can help me to find it?

I'm looking for a dataset of eeg recorded during the pain stimulation (prefered the cold pressure pain stimulation). Anyone can help me to find it?

25 January 2021 301 2 View

Rerfering to somatic symtom disorder: When is a patient preoccupied or simply adequetely worried? How do you define preoccupation in this context?

In order to fulfill criteria for Somatic symtom disorder (DSM 5) a patient needs to be preoccupied with symtoms or their health. Reasonably a symtom like pain does make you preoccupied to some...

19 November 2020 4,195 4 View

What are the 2020 most recommended systematic review and meta-analysis articles on PAIN?

We are starting an investigation, in the field of physiotherapy, on the evaluation and clinic of pain. We are interested in knowing which diagnostic evaluation scales are being evaluated. We...

18 November 2020 2,258 1 View

Multiple regression - What to do with 2 similar independent variables?

17 November 2020 1,938 2 View

ArcGIS 10.2 - Export Dialog Options missing (Option out file format), how to resolve?

I've just unclicked the export map options (advanced options?) and can't get them back. I'd assumed you'd be given the choice of displaying them again, but they are nowhere to be seen. I now can't...

06 November 2020 2,893 2 View

What is the best pain killer for mice?

Good morning, I need to implant chronic electrodes in mice muscles. What is the best injectable painkiller to use during and after the surgery? and what the recommended dose for mice? And for how...

01 November 2020 8,477 1 View