Hi Faheem,

Have you read the recent papers by Scott Buckley and Richard Brackin? They use microdialysis to identify plant available nitrogen in sugarcane plantations. Also see the 2011 paper by Inselbacher et al. regarding microdialysis use to monitor organic and inorganic nitrogen compounds in the soil.

Many Many Thanks Elliot Hill

Please have a look at these links.

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832013000200016

https://www.researchgate.net/post/What_is_the_best_method_to_determine_mineral_nitrogen_in_a_soil_sample

https://www.researchgate.net/post/What_is_a_simple_method_to_determine_total_organic_nitrogen_in_soil_samples

Good luck

Many Many Thanks Arvind Singh.

Nice feedbacks . Here is some; 

Stalk and sucrose yield in response to nitrogen fertilization of sugarcane under reduced tillage  ,Pesq. agropec. bras. vol.48 no.1 Brasília Jan. 2013, http://dx.doi.org/10.1590/S0100-204X201300010001

 Abstract : The objective of this work was to evaluate the agroindustrial production of sugarcane (millable stalks and sucrose yield) after successive nitrogen fertilizations of plant cane and ratoons in a reduced tillage system. The experiment was carried out at Jaboticabal, SP, Brazil, on a Rhodic Eutrustox soil, during four consecutive crop cycles (March 2005 to July 2009). Plant cane treatments consisted of N-urea levels (control, 40, 80, and 120 kg ha-1 N + 120 kg ha-1 P2O5 and K2O in furrow application). In the first and second ratoons, the plant cane plots were subdivided in N-ammonium nitrate treatments (control, 50, 100, and 150 kg ha-1 N + 150 kg ha-1 K2O as top dressing over rows). In the third ratoon, N fertilization was leveled to 100 kg ha-1 in all plots, including controls, to detect residual effects of previous fertilizations on the last crop's cycle. Sugarcane ratoon was mechanically harvested. A weighing truck was used to evaluate stalk yield (TCH), and samples were collected in the field for analysis of sugar content (TSH). Increasing N doses and meteorological conditions promote significant responses in TCH and TSH in cane plant and ratoons, in the average and accumulated yield of the consecutive crop cycles.

Sugarcane Response to Nitrogen Fertilization on a Histosol

with Shallow Water Table and Periodic Flooding ,J. Agronomy & Crop Science (2008) ISSN 0931-2250,doi:10.1111/j.1439-037X.2008.00329.x

Abstract : Sugarcane (Saccharum spp.) is increasingly exposed to periodic floods and shallow water tables on Histosols in Florida’s Everglades Agricultural Area (EAA). In the past, when these soils were usually well drained, they provided excess N for sugarcane through microbial oxidation. It is not known if supplemental N would now improve yields because microbial oxidation is reduced by shallow water tables and periodic floods. The purpose of this study was to evaluate the effects of N fertilizer rates on two sugarcane cultivars exposed to a 25-cm water-table depth with and without repeated 2-day floods. Two studies were planted in containers in 2001 and 2002 with two sugarcane cultivars and five equally spaced rates of N fertilizer from 0 to 200 kg ha)1 . Leaf, stalk and root weights were reduced by periodic flooding and the magnitude of the reduction sometimes differed between cultivars. Plant weights, leaf chlorophyll content (SPAD) and leaf N content were often highest near an N rate of 100 kg ha)1 .  Usually, N fertilizer rate did not interact with water treatment. Nitrogen fertilization may be useful for sugarcane exposed to water-table depths of 25 cm with and without 2-day repeated floods on EAA Histosols.

Thanks a lot Anoop Kumar Srivastava

Dear Faheem Khan

I hope these two papers interesting for you

Dear Dr Khan

I hope that this file can help you

Dear Khan,

Hope the attached paper would be beneficial in your research purpose.