Hello,
I asm currently working with alginate and silk fibroin hydrogels. I prepared a blend of alginate and aqueous silk fibroin (straight from dialysis) and resorted to ionic crosslinking to form firm gels. After 7 and 14 days of culturing (ECs and/or fibroblasts), I found globular domains of SF on the surface of the hydrogels. At the same time, a lot fewer cells a a lower metabolic activity were found on these gels, comparatively to alginate-only. I related this to the conformational change of SF from random coil to beta sheet, that has been proven to kill cells (https://www.nature.com/articles/srep31037 ). However, this was only tested for encapsulated cells. Since mine were cultured at the surface of the gels, I am not confident that this is a suitable explication. I tried to find articles relating to silk fibroin conformational change and cells toxicity/down regulation of cell adhesion, however, I could not find anything. Has anyone heard of a similar phenomenon?
Thank you in advance!
https://onlinelibrary.wiley.com/share/BSCRUV7NACRREJNP83DD?target=10.1002/jbt.22056
Use this, I think that will be useful
Sodium alginate has been studied extensively and found to be GRAS. In fact, in a 1991 study, it acted most as a fecal bulking agent (Dietary effects of sodium alginate in humans, D M Anderson 1, W G Brydon, M A Eastwood, D M, DOI: 10.1080/02652039109373974). However, silk fibroin has had more checkered history with respect to toxicity. Although it has shown promise for TE studies, it can induce cellular and mitochondrial dysfunction (Evaluation of the Toxicity Effects of Silk Fibroin on Isolated Fibroblast and Huvec Cells
Parvaneh Naserzadeh, Seyed Alireza Mortazavi, Khadijeh Ashtari, Enayatollah Seydi, and Jalal Pourahmade, Iran J Pharm Res. 2018; 17 (Suppl2): 134–145).
- Badges
- Science topic
- Similar topics
- Gels