Aldose reductase inhibition Assay protocol

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Adam B Shapiro

Look for methods in publications in which people report screening for and/or testing the potency of aldose reductase inhibitors. Here are some examples.

http://pubs.acs.org/doi/abs/10.1021/ci400322j?journalCode=jcisd8

https://www.ncbi.nlm.nih.gov/pubmed/19301313

http://www.sciencedirect.com/science/article/pii/S0014579309008266

Nirmala Chinnappareddy

Dear Adam

Thank you very much for taking your time to answer my question. I am following the same protocol mentioned in these papers. Unfortunately I am not able to detect any enzyme activity in my reaction mixtures. Just wondering if anyone has a working protocol to share in detail.