How long can hold alcoholic (ethanol) extract of plant for assessment of proline and soluble sugars in plant and at what temperature؟
Dear Elham Maghsoodi
Melting point 205 to 228 °C (401 to 442 °F; 478 to 501 K) (decomposes)= https://en.wikipedia.org/wiki/Proline
Pls. find the attached files
https://www.researchgate.net/publication/43132146_Methods_for_Determination_of_Proline_in_Plants
http://link.springer.com/article/10.1007/BF00272538
http://www.omicsonline.org/open-access/a-review-of-extraction-and-analysis-methods-for- https://www.researchgate.net/publication/211353600_PROTOCOL_Extraction_and_determination_of_proline studyingosmoregulants-in-plants-2157-7064-1000315.pdf
http://www.sciencedirect.com/science/article/pii/S0570178316300021
http://www.nrcresearchpress.com/doi/10.1139/cjps-2015-0372
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300004
http://prometheuswiki.publish.csiro.au/tiki-index.php?page=Extraction+and+determination+of+proline
page 263 in: Akshay Kumar Chakravarthy. 2015.New Horizons in Insect Science: Towards Sustainable Pest Management Springer,Pp.431. ISBN8132220897, 9788132220893
http://aob.oxfordjournals.org/content/89/7/813.full
http://www.publish.csiro.au/fp/PP9740087
http://www.sciencedirect.com/science/article/pii/S0308814608004226
http://www.nrcresearchpress.com/doi/abs/10.1139/cjps-2015-0372#.WAZw5jZWH2s
http://www.sciencedirect.com/science/article/pii/000326979290285F
https://www.researchgate.net/profile/Vladimir_Kuznetsov12/publication/276268327_Kuznetsov_Vl.V._Shevyakova_N.I._Stress_responses_of_tobacco_cells_to_high_temperature_and_salinity._Physiol_Plant_1997_100_320-326/links/5553872008ae6943a86f2b8b.pdf
Hoping this will be helpful
Regards
Prof. Houda Kawas
Article Methods for Determination of Proline in Plants
Article PROTOCOL: Extraction and determination of proline
Data Kuznetsov Vl.V., Shevyakova N.I. Stress responses of tobacco...
Dear Elham Maghsoodi
The more you have water in your extract, the less the extract wil remain stable. Whatever the concentration of ethanol is, it is more suitable to store it in cool (preferably negative) temperature. Alternatively, the extract may be freeze-dried if to be stored for a long time.
you could store your alcoholic extract of proline and sugars for weeks at a temperature of -18 C.we have used this procedure to store alcoholic extracts of amino acids , sugar s and plant hormones for at least 8 weeks.
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