Conducting a survey to understand the response to work from home during the covid-19 lockdown?

Greetings, We are conducting a survey on work from home and its impact, please fill the questionnaire and contribute to the growth of academic understanding of the concepts and for policy...

03 May 2020 3,758 22 View

What are recommended temperature conditions for purifying his tagged proteins under denaturing conditions(GuHcl)?

Hello everyone, I'm trying to purify his-tagged protein under denaturing conditions using Guanidine Hydrochloride (Protocol from GE Healthcare-attached below). Though I'm getting my protein in...

10 December 2019 8,654 3 View

How to access stress/ strain at integration points in a umat code?

Im trying to code a damage model for beam elements, for which I know the strain at rupture. I was wondering if I could access the strain at the center of the beam to start and progress damage. As...

28 May 2019 9,755 1 View

Can any one help me to get the MATLAB code for cluster head selection and routing in wireless sensor networks using PSO and GA?

wireless sensor networks- optimization techniques

10 May 2019 2,944 1 View

How much is 1 volume of 70% ethanol?

I need to isolate RNA from the tissue sample. The procedure is given like "add 1 volume of 70% ethanol to the lysate, and mix well by pipetting". How much ethanol should I add?

19 November 2017 8,272 5 View

Bacterial intracellular synthesis of nanoparticles,Could anyone suggest any standard bacteria(preferably gram negative bacteria)for NP synthesis?

Hi, this is part of designing a practicles for students, could anyone suggest any bacteria mediated intracellular synthesise of any nanoparticle (10-50nm) and that gives highly repeatable...

13 December 2016 3,835 4 View

What is the method for separate out the mixture of Ethyl acetate and tetrahydrofuran ?

30 June 2016 9,968 9 View

What are the possible causes of piston or gudgeon pin failure?

Piston pin is subjected to both shear and bending loads. I need some clarity in this scenario to complete my paper. The system runs at a wide range of RPMs , so fatigue also may be one of the...

04 March 2015 4,858 4 View

Why do I get two bands after digesting my plasmid with PfoI?

I have a vector with PfoI site. I did single enzyme digestion to check for the linearity. For the reaction, I used 5U of thermo scientific PfoI for 1ug of plasmid with an incubation time of...

22 December 2014 2,981 7 View

Will my specificity of Restriction Endonuclease BSU36I be affected?

I am working on a gene which is cloned in a vector which has an internal restriction site for BSU36I. The actual recognition sequence for BSU36I is 5' CCTNAGG 3', and the my gene has 5' GCTCAGG...

17 July 2014 5,372 2 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

Where to find data on trees in tropical agroforestry?

Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...

28 February 2021 7,356 8 View

How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

28 February 2021 9,464 2 View

Good morning research fellows, Has anyone worked with senna alata plant extract on any kind of fish?

Literatures on the piscicidal effect of sennaa alata is scanty. if you have any related litrature either in the bark, leaves or roots used as treatment to any aquatic organism please kindly help...

28 February 2021 9,138 2 View

The Shannon's index is inversely proportional to Simpson's index (1-D)?

I'm having a problem with my bloxplot with Shannon's and Simpson's indexs. The value of Shannon for my Community 1 indicating higher diversity (in relation of the Community 2), but have lower...

26 February 2021 3,033 9 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View