Do mature (virgin) forests take up carbon, if there is no net increase in biomass?

I seem able to get any answer I please just by searching a little more. Where is the truth? Concerning untouched Amazon rain forest, when people measure carbon flux, do they include the carbon...

27 October 2020 2,546 4 View

If one wants to link together power sources all across North America, would it be better to use DC transmission lines, or gas pipelines, say with H2?

Intermittent renewable energy sources become more efficient and reliable as the number and diversity of sources is increased. But electrical transmission over distances greater than 2000 miles...

20 October 2020 731 5 View

Using antimicotics such as Nystatin or amphotericin B in mouse neuronal cultures?

Hi all, I just started to prep mouse cortical neutrons for E16.5 and in my second prep I had a massive fungal contamination. Since these preps are quite precious, I wonder if I could add an...

15 September 2020 645 1 View

Is it possible to add a selection agent in using pCAGGS for transfection of HEK-293T cells?

I will try to express proteins in HEK-293T cells. I had previous experience in another cell line where I used co-transfection for selecting transfected cells. I had observed lower levels of...

16 May 2020 2,857 1 View

Does anyone have a general Hydrophobic Interaction Chromatography Protocol?

I have a sample suspended in 0.34 M NaCl after using ion exchange chromatography. I would like to perform hydrophobic interaction chromatography (HIC). Is it okay that I use greater salt...

27 August 2019 8,417 1 View

What is the difference between the columns Hi Prep 26/60 S-100 HR and Hi Prep 26/60 S-200 HR?

I am trying to have a better resolution during purification. Would running my sample using S-100 HR give a better result from an S-200 HR run? Also, other than the fractionation range, what is...

24 July 2019 4,378 3 View

Exemplary models of elementary school speaking-listening skills group instruction-assessment: Successful school sites and teachers?

School districts make public claims and governmental contracts regarding essential English Language Arts Speaking skills, however transparency and accountability is lacking about implementation....

22 January 2019 2,420 3 View

For EV development, what is a good number for battery energy density?

For future EV design, I have tentatively chosen 0.157 kWh per kg. for energy density of the battery pack. Am I aiming too low for a vehicle likely to reach production in five years? Or, is my...

27 December 2018 2,368 1 View

Why the pH of xanthan gum in aqueous solution is 7 (neutral) when it has glucuronic and pyruvic acids in its chemical structure?

I find it pretty confusing since xanthan gum has both glucuronic and pyruvic acids in its chemical structure, it should release H+ ions upon solvation in an aqueous solution and make it acidic.

18 January 2018 9,190 2 View

Does anyone observe decrease in growth rate of Drosophila S2 cells in serum-free media?

I have adapted my Drosophila S2 cells growing in complete Schneider's medium to serum-free media. After a month of passage in serum-free media, the cells continually exhibit decreased growth rate...

04 December 2017 9,887 2 View

Hi i want to ask that in order to find out LC 50 (96 hours) of particular toxin for fish. Should we keep the animals with food or without food?

if we expose the fish after acclimatization to particular toxin in order to find out LC 50 (96 hours).Should we keep the fish off feed or with feed for that duration. Some literature show that...

24 February 2021 357 2 View

Does anyone have any ideas why drug-resistant malaria is increasing in Southeast Asia even when those drugs are no longer used?

When dealing with bacterial toxins, there are three varieties of microbes that develop in your petri dish: toxic, resistant, and susceptible. Initially, there are only toxic and vulnerable...

21 February 2021 6,084 5 View

How to dilute Toxin-DMSO solution?

I have a concentrated solution of a toxin(50 micromolar) in DMSO 100%. I need to dilute it inorder to dilute both the toxin and DMSO wich can be toxic to cells. Which would you guys recommend to...

19 February 2021 7,447 2 View

Can someone help me to improve the MCF-10A growth?

18 January 2021 7,640 3 View

Can i run native agarose gel electrophoresis in TAE buffer?

I am characterizing different snake venoms. As snake venom is a mixture of various large protein complexes (with both acidic and basic proteins) i want to run native agarose gel electrophoresis...

07 December 2020 4,629 3 View

How to dye the spider gland of a spider to see in fluorescence (488nm)?

I want to use a fluorescence microscope to observe in situ the venom gland of a cleared spider using a 488 nm laser. Any recommendation on what dye to use that would be particularly attached to...

14 October 2020 3,986 10 View

Is there anyone who can help me to learn Venom Proteomics?

I am interested to do my PhD research on venom proteomics. I don't have details idea of it. I am a beginner in this area. Is there anyone who can support me to provide necessary information...

03 August 2020 1,820 3 View

How can I detect the presence of antibodies in plasma using electrophoresis?

Can we use PAGE to detect presence of antibodies in plasma? I am planning to characterize antibodies after immunization of mice with whole venom. How can I purify the plasma to get rid of other...

30 July 2020 3,400 1 View

How to extract polygons of species distribution from books?

If you need a distribution map from a book, how do people deal with that? Do u email authors to obtain the layers or is there a way to export the polygons from the book images? Colleagues and I...

20 June 2020 4,391 1 View

Where to find predator:prey mass data (for terrestrial carnivores)?

I'm looking for datasets (or databases) in which the body mass of predator and prey are presented. I'm specifically interested in terrestrial predators (snakes, mammals, birds). An example of is...

16 June 2020 2,453 9 View