Not knowing why Mr. Abhinav Singh proposes the ERLANDSEN et al 2004-Paper (BTW: the open Access Article is available at: Article High-resolution Visualization of the Microbial Glycocalyx wi...

= Article High-resolution Visualization of the Microbial Glycocalyx wi...

(due to linking always to the article at the RG-site, the altered original web-location: = https__://journals.sagepub.com/doi/10.1369/jhc.4A6428.2004 ().

PDF at:Article High-resolution Visualization of the Microbial Glycocalyx wi...

=> https__://journals.sagepub.com/doi/epub/10.1369/jhc.4A6428.2004

(COPY and PASTE into your browser, again: before executing that link-address in your browser: delete the underlines in between https __and __ ://journals.....).

The Keywords in that article tell 'only': Keywords

glycocalyx, polycatioic dyes, alcian blue, safranin O, low-voltage SEM, Enterococcus faecalis, Klebsiella pneumoniae, exopolysaccharides....nothing said about lipid vacuoles - fat- adipose [adypose?] tissue or optimal embedding into EPON.

Dear Mr./Dr. Francesco Boldrin - it would help a lot if you could post / add in this your Question / request a .docx or a .PDF enlisting the specimen-prep.-steps you are following up to now... for the experienced it will be a lot easier to follow the possible problems AND to solve the matter / propose a suitable protocol...BTW: animal / human tissue?, location (e.g.: internal organs, skin, ...) size of specimens demanded/as may be necessary? And last but not least: since there are different types on the market: which EPON (previously / in earlier times brand/TM of Monsanto...) resin are you using (brand)? I am willing to chime in, if you allow / have no objection (;-)) Cordiali saluti, W.H.M., Salisburgo, Austria

Me too..