Ok so I've been maintaining Eucalyptus species callus cultures, and have run into issues with phenolics, so I've tried to make media (MS) with 1g/L activated charcoal, but it seems that the AC has reduced the gelling capability of the medium substantially. The medium is semi-solid (I used 4g/L agar to keep the medium a little squishy but I didn't want it THAT squishy
any tips? Maybe add a little KOH to balance the pH and improve the agar's gelling capacity?
Dear Alex Baranowski I never before observed this effect with AC, but it happens with other supplements. In any case, you must to consider it, and increase a little the agar concentration to obtain the desired consistency
Dear Alex Baranowski I have worked many years with AC and I have never seen this effect on the culture medium. I agree with Ricardo Julian Licea-Moreno . You should add a little more agar and you will get the consistency you want. Make sure your medium pH is adjusted.
Good luck.
Addition of activated charcoal doesn't affect pH that much but it's better to keep a bit high pH (6.0) before autoclaving. I guess the agar 4 g/l is less you can increase it to 7g/l. You should stir the flask before autoclaving because ac has solubility issue and sometimes it will sink at the bottom of your flask and get solidified there. Also avoid using expired ac bottles.
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