I am trying to simulate phase change of refrigerant inside pipe extracting heat from panel in comsol. Can anyone help with how to simulate this?

My model is a rectangular multi layered structure under which refrigerant flows through cylindrical pipes. A heat flux of 600W/m2 applied on panel and internal forced convection is used for...

20 January 2021 9,478 1 View

Why does the ghost atoms gives error in NICS calculation with wb97xd in gaussian09 ?

In a trial, I am trying to calculate NICS (0 and 1) values for benzene ring using gaussian09. I have placed the ghost atoms at the center, 1A, and 2A above the rings. On using the method/basis...

19 January 2021 3,188 3 View

Rejected manuscript for journal publish after 1st revision, what needs to be done next?

Hello, My manuscript has been rejected by the editor and reviewers even after the 1st revision. Though I tend to disagree some of the points raised, assume I can't request for another revision...

28 December 2020 6,062 3 View

What is the actual basis on which amount of Perovskites materials are to be taken?

In some discussion people took 112 mg of MAI and 461 mg of PbI2 in 1 mL DMF and some took 461mg of PbI2 too but 159mg of MAI there are many other authors took different amount of these materials...

17 September 2020 9,998 3 View

Forums to get online survey responses?

For my journal publish, I have been asked to increase the sample size by 200. I have been individually contacting more than 1000 Linkedin contacts and posted in all professional groups. But have...

04 September 2020 2,564 5 View

Derivation of Rice and Tracey equation???

12 July 2020 6,555 1 View

Can anyone suggest me where to get a low cost 2D Heterostructure Transfer System?

Except the 2D transfer system sold by HQ Graphene

09 July 2020 3,276 4 View

Why do mangroves intake oxygen through aerating roots like pneumatophores?

Many mangroves like Avicennia and Sonerratia species have developed pneumatophores as an adaptive strategy to survive in hypoxic environment. These pneumatophores receive oxygen from the...

16 April 2020 2,203 3 View

What is a popular book for understanding laser fundamentals and evolution and types of lasers?

I looking for books, channels, videos and PDFs regarding laser and particle interaction and evolution of laser power and laser types and how laser makes the physical changes on the ceramic...

05 April 2020 3,396 25 View

Why band gap of exfoliated WO3 nanosheets is less than bulk WO3?

Usually if we go from bulk (3D) structure to 2D, 1D, 0D structure, band gap of the materials increases accordingly. But, in my case exfoliated WO3 ( expected to have 2D sheet like structure/...

25 March 2020 9,183 7 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View