According
Average 3 period
As flaviviruses genome is very frequently prone to mutation hence it is very difficult to clone whole genome inside vector what kind of vector and cells can sustain such amount of cloning...
11 July 2024 4,284 0 View
I am working on cloning of a gene fragment inside my vector everything we tried to do correctly like making insert and vector with utmost care but after ligation the morphology of colonies seems...
11 July 2024 9,010 3 View
I have made changes in .in1 as well as in .int files but unable to get dos till 20 eV
13 June 2024 9,080 1 View
phenol chloroform extraction method is used for purification of nucleic acids DNA/RNA
03 April 2024 8,268 0 View
phenol chloroform extraction method is used to purify nucleic acids to obtain a good concentration.
03 April 2024 7,244 0 View
as viruses are notorious for being prone to mutation at high rates its very difficult to clone them though general cloning procedures and cells so in order to solve this problem need specific cell...
03 April 2024 9,031 5 View
For the boltaztrap we used the formula for calculating the Seebeck coefficient using a different formula mentioned in doi:10.1016/j.cpc.2006.03.007 and the formula we are using in the experiment...
17 March 2024 2,105 3 View
There are two rooms: Room A and Room B; initially both are dark; contains no light source (of whatsoever). Now, I bought a light source and set it up in the room A, enabling me to see things...
28 February 2024 4,809 10 View
I am performing snake venom fractionation using Reverse-phase HPLC. It generates multiple peaks with different area under the curve. Since I'll be analyzing multiple chromatograms at different...
08 November 2023 9,134 0 View
Dear All, Biotechnologists from The area of Biotechnology. Currently I am serving as HoD in Department of Biotechnology, IMSEC, New Delhi NCR. Is there any person who can help me for two or three...
08 November 2023 2,785 4 View
Could you recommend some articles on Urban Transportation System optimization and Innovation?
13 August 2024 2,595 3 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
average particle size calculation from TEM
04 August 2024 2,921 1 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View
Hello everyone What is your opinion about the introduction of an expert decision support system in which the rules are extracted from existing data without human intervention, instead of being...
31 July 2024 5,785 4 View
After a lot of feature engineering for click-through rate modeling, it feels like it's basically the end of iteration? I mean, it's not cost-effective to keep doing it?
29 July 2024 4,955 0 View
