is it possible to accelerate the composting proccess by adding Active Microbial/Bio Fertilizer (e.g. Azetobacter or Pseudomonas) ? How efficient would it be?
Conversion of spent mushroom substrate to biofertilizer using a stress-tolerant phosphate-solubilizing Pichia farinose FL7https://doi.org/10.1016/j.biortech.2012.02.042Get rights and content
Abstract: To develop high-efficient biofertilizer, an environmental stress-tolerant phosphate-solubilizing microorganism (PSM) was isolated from agricultural wastes compost, and then applied to spent mushroom substrate (SMS). The isolate FL7 was identified as Pichia farinose with resistance against multiple environmental stresses, including 5–45 °C temperature, 3–10 pH range, 0–23% (w/v) NaCl and 0–6 M ammonium ion. Under the optimized cultivation condition, 852.8 mg/l total organic acids can be produced and pH can be reduced to 3.8 after 60 h, meanwhile, the soluble phosphate content reached 816.16 mg/l. The P. farinosewas used to convert SMS to a phosphate biofertilizer through a semi-solid fermentation (SSF) process. After fermentation of 10 days, cell density can be increased to 5.6 × 108 CFU/g in biomass and pH in this medium can be decreased to 4.0. SMS biofertilizer produced by P. farinose significantly improved the growth of soybean in pot experiments, demonstrating a tremendous potential in agricultural application.
- Bioresour Technol. 2012 May;111:410-6. doi: 10.1016/j.biortech.2012.02.042. Epub 2012 Feb 17.Conversion of spent mushroom substrate to biofertilizer using a stress-tolerant phosphate-solubilizing Pichia farinose FL7.
- Abstract: To develop high-efficient biofertilizer, an environmental stress-tolerant phosphate-solubilizing microorganism (PSM) was isolated from agricultural wastes compost, and then applied to spent mushroom substrate (SMS). The isolate FL7 was identified as Pichia farinose with resistance against multiple environmental stresses, including 5-45°C temperature, 3-10 pH range, 0-23% (w/v) NaCl and 0-6M ammonium ion. Under the optimized cultivation condition, 852.8 mg/l total organic acids can be produced and pH can be reduced to 3.8 after 60 h, meanwhile, the soluble phosphate content reached 816.16 mg/l. The P. farinose was used to convert SMS to a phosphate biofertilizer through a semi-solid fermentation (SSF) process. After fermentation of 10 days, cell density can be increased to 5.6 × 10(8)CFU/g in biomass and pH in this medium can be decreased to 4.0. SMS biofertilizer produced by P. farinose significantly improved the growth of soybean in pot experiments, demonstrating a tremendous potential in agricultural application.
Dr. Arifin, what mushroom substrate you are talking? The decomposition and acceleration of the process depends on the the substrate you are using for the growth of mushroom. In general, among the cereal straw wheat, rye and oat are good for mushroom growth. But problem is it should have heat pasteurized as it laden with other microbes that may compete with mushroom mycelium growth. You may use Log such as Elm, beech, alder, cottonwood etc for mushroom growth. For commercial growth people are mainly using enriched sawdust. Sawdust always needs to be enriched with nitrogen supplement such as bran. It is better to sterilize saw dust before use. The other effective substrate are Stumps, compost, paper&paper products, Cardboard, coffee grounds, tea leaves, gardening debris etc.
The decomposition based on the C/N ratio of the substrate used and the composition of the substrate. Accordingly, the time will taken for decomposition and you may decide the inoculation material in the substrate. If C/N ratio of your substrate is very wide then you have to add N rich material to reduce the C/N ratio so that you can minimize the decomposition time. Similarly, if your material have more lignin, hemicellulose or cellulose compounds then you may inoculate some cellulase, hemicellulase or lignase secreting organisms and so on.... But be sure you have enough moisture ( at least 50% WHC) and optimum temperature (28-32 degree Celsius) during the decomposition process.
I am talking about A.bisporus Substrate and mostly consist of Paddy Straw.
Thank You for your answer Dr Tarafdar
Dr. Arifin, the C/N ratio of substrate like paddy straw is generally 51:1. So, for faster decomposition you have to add some N may be urea or organic substances like Chicken manure (C/N ratio 9.6;), Pugo (C/N ratio 6.7), or night soil (C/N ratio 6.7) so that your C/N ratio of the paddy straw drop down to 30:1 for faster decomposition. You may also inoculate efficient cellulase, hemicellulase and lignase producing organisms. But dont forget to maintain moisture level and temperature as mentioned earlier during decomposition.
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