What Taxonomy for these acica seeds
How can I determine a good adhesion strength range for coatings on polymer surfaces, such as DLC on polymer substrates? Is there a specific threshold for adhesion strength (from T-peel tests)...
10 August 2024 942 3 View
What is the method for analytically calculating the wear and service life of polymer-polymer sliding pairs?
04 August 2024 1,078 1 View
If the pairs are similar, will it reduce the adhesion due to electric charges?
29 July 2024 3,185 2 View
I've been trying to design primers for qPCR to detect NCF1 cDNA using Primer3Plus and NCBI Primer-BLAST, but I couldn't find primers that are specific to the gene. Any help ? Thanks !
04 July 2024 3,068 3 View
I am trying to understand the influence of polymer films sliding against each other. For the simulation, I saw many molecular dynamics simulations, like LAMMPS, GROMACS, etc. I am new to this...
09 April 2024 8,871 2 View
Hello, I am using dinitrophenol and rotenone treatments on healthy fibroblasts to study cell signalling pathways (ISR and UPR). I have chosen 1mM and 500uM based on other work but have not had...
25 March 2024 1,414 0 View
I have been trying for months to grow kidney cells from rat kidneys, I tried several methods, serial sieving, cutting and meshing, and using kidney tissue to obtain an outgrowth of cells. I always...
11 March 2024 2,665 1 View
if I have 5 grams of PMMA, how much (mL) of Chloroform should I used to dissolve it ? should U use sealed beaker to this process?
25 February 2024 9,066 0 View
Hello, Does anyone have any idea of the exact (if any) number of cells we can have in mosquito salivary glands (the species doesnt matter at this stage). Thank you
05 February 2024 823 0 View
Hi how can I upload a manuscript for a paper that wasn't uploaded before thanks?
21 December 2023 7,777 0 View
I'm working on camelina seed sterilization and have some doubts about the process. I followed a protocol used by my lab mate, but I'm unsure about a couple of things: Is it safe to use 10% bleach...
02 August 2024 1,178 3 View
I have been following Rivero et al 2014 protocols, but I am not sure when I should stop watering because my plants are completely yellow but the siliques are not 80% brown. Any recommendations?...
30 July 2024 2,301 7 View
Hello all, Recently as part of a research project at university, we have been studying the effects of light and temperature on eucalyptus seed germination. In our initial run of trials we...
24 July 2024 8,850 4 View
Hi, Kindly guide me that how many cells of HEC1A & HEC1B Cell lines should I seed for Wound healing assay and which plate type is recommended 6, 12 & 24?. Articles suggested mainly 24...
20 July 2024 4,143 2 View
I used MDCK cells for my plaque assay for influenza virus and seeded 950,000cells/well in 6-well two days before infection. The virus was diluted in FBS-free MEM with 0.5ug/ml TPCK-trypsin. Here...
11 July 2024 5,669 5 View
I have protein percentage and Seed yield per hectare data, how to calculate protein content kg per hectare.
11 July 2024 9,045 4 View
I am currently testing the effect of bacterial filtrates on cancer cells , after seeding the cells I tested the bacterial filtrates against them , and got images of all 96 wells using inverted...
10 July 2024 7,145 2 View
I seed my cell (KG1a )in opti-mem media for transfection (without lipofectamine as control ) and after 2 hours I add FBS 10 ul in each wells all my cells after 24h in control wells or with...
07 July 2024 407 0 View
I have attached pics of the SH-SY5Y cells within a seeded plate after reaching >80-90% confluence. Are these dark clumps of multiple growing cell layers? Or dead cells? Any clue? many thanks.
01 July 2024 8,361 3 View
Hi everybody, i need to pick your brains: i work with an adhesive fish cell line which forms a konfluent monolayer. I want to test an adsorbant substance, therefore i want to saturate the cell...
30 June 2024 9,300 0 View
