I was trying to measure antioxidant activity from bacterial crude extract. However, the absorbance of sample+DPPH is greater that absorbance of DPPH, therefore, I could not calculate the result. Is it unusual to get the result like this? since there was no problem with positive control (VitE).

My samples are colorless, very pale blue and very pale orange, but all the samples give similar trend of absorbance.

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