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Questions related from Natthaporn Takpho
I'm working on Sanger sequencing using Bigdye V.3.1 and purify the sequenced product by DyeEx 2.0 kit. The chromatogram was fine and readable, however, it always contains some noise background and...
20 November 2017 2,506 10 View
I have tagged my interested protien with GFP to observe the localization one of them can be perfectly observed but another 2 did not? I wonder that what's wrong with them since there is no problem...
01 February 2017 9,036 5 View
I have studied about the mitochondrial enzyme in yeast. So, I want to extract the crude enzyme from cell culture in order to test the enzyme activity. What is the suitable procedure in this case?...
01 September 2016 6,369 3 View
I'm doing the gateway cloning from PCR product. I first ampliflied my interest gene with the attB1/2 and then performed the BP clonase reaction to construct the pDONR211 vector with my inserted...
18 April 2016 8,750 6 View
I'm doing PCR with primer tagging with attB1 and attB2 sequences for gateway cloning system. My target gene was ligated to pRS416 plasmid, 2 kb and 1 kb in sized. I'm using KOD FxNeo PCR kit and...
02 January 2016 7,105 14 View
I've performed URA3 deletion in S. cerevisiae S288C by KANMX6 replacement. Transformants are able to grow on YPD containing 150 ug/ml G418 but not for the control (S288c). These transformats...
26 March 2015 5,206 7 View
I have introduced 1 base mutation which cause 1 amino acid changed by PCR then transformed this plasmid to DH5-alpha. At this step, I checked desire transformants byspread on selective media...
17 December 2014 3,640 3 View
I wonder how can we utilise the result from microbiome analysis into functional cluster grouping by ability to produce enzymes, secondary metabolites and etc. For example, soil microbiome analysis...
01 January 1970 1,739 5 View
