Indian Nutmeg oil contains 3% myristicin. Is this the % that we get directly from GC without internal or external std?
No % will only calculated based on internal standard. So procure myristicin and measure using GC or HPLC
Dear Sanjeev,
I was also believed on internal std calibration. But please see the attachment. it is BIS standard for palmarosa oil. In that the graniol content is determined by GC analysis. it is clearly written as direct GC%. I dont understand this. Also as a note: they had given Internal standardization can be used if pure appropriate internal standard is available. That means if IS not available then we can take direct GC%?
Check that your answer is whether in your linear range of concentrations. You do your calibration, your curve and you verified that it is a straight (coefficient of correlation> 0.9xxx).
In general, to validate a method, you have to check the reproducibility, repeatability, linearity, limit of detection and quantification limit ... I do not think I did forget.
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