Abnormal peak shape in SEC?

More Meiyue Wang's questions See All

Do you think can be any Uranium bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about Uranium ore deposits in world.

11 August 2024 6,718 0 View

Do you think can be any diamond bearing rocks in Eastern part of Iran and western part of Afghanistan?

I want to know more about diamond ore deposits in world.

11 August 2024 2,165 1 View

If Banks do not provide credit facility, what are the options available for FPOs and impact on producer’s income?

10 August 2024 8,197 5 View

What is the difference between mathematical R^4 space and physical 4D unit space?

We assume that the difference is huge and that it is not possible to compare the two spaces. The R^4 mathematical space considers time as an external controller and the space itself is immobile in...

10 August 2024 6,676 14 View

What are a “Farmers Producer Organization” (FPO) and its essential features?

10 August 2024 475 5 View

Controlling for pupil light reflex when analyzing pupil size time course?

I used eye tracking to examine how participants from two different populations (A and B) react to an image. Participants in population A exhibit larger pupil sizes over time, but they also have...

10 August 2024 3,226 0 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,537 5 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,286 2 View

How to get moment output in Abaqus Standart?

I have input a moment load in module load Abaqus, i put my moment load on the node surface (using reference point). I have define moment in history output and make a set for moment too. But the...

08 August 2024 4,828 4 View

How is energy cycled through the Earth's climate system and how do matter cycle and energy flow through the rock cycle?

08 August 2024 8,159 0 View

A positively charged and 10x his-tagged protein that doesn't bind to any chromatographic resin?

Hello everyone, I am currently working on a protein that is 10x his-tagged and positively charged (predicted pI=10.16). But when I tried to use Ni-NTA column to purify the protein, it's not...

24 July 2024 7,292 4 View

Recommendations for his-tag magnetic beads / resins?

Good day everyone, Trying to perform protein purification for the first time, gotta buy the whole equipment/reagents. Protein of interest 48 kDa (Antithrombin), expressed from his-tagged plasmid...

16 July 2024 3,691 6 View

How to identify a monomeric protein species of the target without running the molecular weight standard on SEC?

I was using Superose6 10/300 Gl column for purification of my target protein. the sample consists of various oligomeric state and i would like to identify the approximate elution volume where my...

15 July 2024 5,156 2 View

Can I detect the FLAG sequence of my AAV vector by qPCR?

I am treating human cells with an AAV vector encoding for my gene of interest tagged by FLAG. I want to quantify the mRNA expression of my gene of interest via qPCR to discriminate the endogenous...

14 July 2024 8,479 3 View

Is the wash in my IP the problem or something else?

The image shows 2 western blots. The first one is of protein A, and the second is for the flag tag that the protein is bound to. The lanes are as follows Ladder, Beads, SAB(unbound), and elution....

01 July 2024 3,183 0 View

Why does my protein bind poorly to the Ni sepharose beads?

I am purifying RNAse E in E. coli (118 kDa). I cloned the rne coding sequence on pET28a with a His tag at the N-terminus. The protein overexpressed fine (see attached figures). The binding buffer...

24 June 2024 6,287 4 View

How to predict Protein structure and choose N or C terminal tagging?

Hi, may I know I want to fuse my protein with affinity tag for protein purification in Expi293, how can I determine and visualise how does a N or C terminal tag affect my proteins (structure,...

24 June 2024 8,620 6 View

Is there any equipment that replaces GPC/SEC in carrageenan characterization?

22 June 2024 2,361 3 View

Minimum concentration of protein to be injected for detectable peaks of protein in 16/60 Sephacryl S-100 HR column for SEC-FPLC?

Hello Peers, Warm wishes to all. I am a new user of the Cytiva AKTA Start system for SEC with a column, HiPrep 16/60 Sephacryl S-100 HR (120 ml column volume). I am not sure about the minimum...

03 June 2024 7,713 2 View

Is Anti-FLAG® M2 Magnetic Beads work for purification of 3x FLAG tagged protein ?

Can anyone share their experience of working with Anti-FLAG® M2 Magnetic Beads used for purification of 3x FLAG tagged protein.

25 May 2024 7,522 1 View

Adam B Shapiro

Three non-exclusive possibilities are (1) the protein is impure, (2) the protein is highly aggregated, and (3) the protein has an affinity for the gel filtration resin.

Deeksha Sugunan

I agree with Mr. Shapiro, Multiple peaks are result of impurities, aggregation- protein existing in multimeric forms.