Deeksha Sugunan

6 Questions 7 Answers 0 Followers

Questions related from Deeksha Sugunan

CD spectra query?

Hello all, I ran CD for my wild and mutant protein but the conc. of both the protein was different. Does this effects the result? when I ran the data in Bestsel software the percentage beta...

13 November 2024 3,025 1 View

Less substrate signals towards the last lanes of the Native PAGE?

I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...

18 July 2024 6,337 4 View

Positive clone but sequencing results showing scattered alignment. reason?

Hello all, I am doing point mutation using overlap methods. I got my overlap , transformed to vector got positive colony pcr result as well. Isolated the plasmids again did pcr with the plasmids...

29 January 2024 5,855 4 View

No PCR bands for my 360 bp target?

Hello all, I am trying overlapping mutation for a site specific point mutation ( I tried with QuickChange kit, not working) . For which I have to first generate two fragments of my target gene....

30 December 2023 8,829 3 View

I am getting less foam in my bacterial cell culture harvest after IPTG induction. reaction conditions are 37 degree for 4 hours after IPTG. reason?

Previously while doing induction, I use to get a layer of froth. but now froth is totally absent and even the colour of LB after induction s not the same. I changed LB, IPTG,antibiotics everything...

17 July 2023 5,789 2 View

Query regarding DNA binding assay - EMSA?

I am doing an EMSA of my protein of interest and a Holliday Junction. the expected outcome is that the lane having the protein DNA complex should have less free DNA but in my case if it shows a...

27 May 2022 7,970 0 View