Meiyue Wang

6 Questions 3 Answers 0 Followers

Questions related from Meiyue Wang

Abnormal peak shape in SEC?

Does anyone know why the peak of purified his tag protein look like this?

03 August 2022 5,210 2 View

Why people also detect phosphorylation of AKT substrate when they used AKT inhibitor?

i have a question (maybe stupid). There is an allosteric inhibitor of AKT (MK2206) which shows highly inhibition on p-AKT (T308 and S473). i think this could indicate that the inhibitor works. i...

09 July 2020 5,306 2 View

How long should i transfer a 500 kda protein for BN PAGE?

Does anyone have the experience with transferring a large protein for BN PAGE? i an working with BN PAGE now. i tried to do transferring at 30v for 3h. but i did not get any band by WB. could...

24 May 2020 7,100 0 View

How to know whether an antibody is phospho specific or not?

I made an phospho specific antibody. I checked this antibody using KI cell lines. there is pan protein but no signal with phospho antibody. i thought it means my phospho antibody is specific. then...

14 May 2020 9,799 5 View

Does phosphorylation of an enzyme affect its Vmax and Km?

Phosphorylation of a protein or enzyme affects its activity most by inducing conformational change of this enzyme. Does anyone know if phosphorylation could affect Vmax and Km?

06 May 2020 2,033 5 View

How to explain an enzymatic reaction produce less product in longer reaction time?

I am doing enzymatic reactions recently and then check the amount of product in the end by HPLC/MS. The wired thing is that reactions with shorter reaction time show higher amount of product. It...

17 April 2020 7,982 7 View