How to increase the final DNA yield using the Qiagen PowerSoil DNA extraction kit?

Hi, I am having troubles trying to extract DNA using the Qiagen PowerSoil kit from cave sediment (mud/clay). I always get very low yield:

03 January 2021 5,049 1 View

Can all iron oxide nanoparticles be considered superparamagnetic?

Dear researchers, Can all iron oxide nanoparticles be considered superparamagnetic? Or the "superparamagnetic" definition can be assigned only to certain types of iron oxide nanoparticles? If so,...

26 October 2020 546 5 View

Do you know how long it takes for a polyprepylene surgical mask to degrade?

I'm a master student in the university of Geneva. I have to find a scientific paper of the degradation of the surgical mask to add it in my master's thesis. I only found non-scientific journals...

03 September 2020 2,940 3 View

Piecewise multiplicative scatter correction in R?

Anyone is aware of an R package available online to perform a Piecewise Multiplicative Scatter Correction (PMSC)? This technique is an advance of the Multiplicative Scatter Correction (MSC)...

23 August 2020 2,893 2 View

What is the best chromatography (type, resin..) to separate blood lipoproteins among each other?

Dear all, I am trying to understand which is the "golden standard" method to separate lipoproteins from the blood (LDL, HDL, IDL, chylomicrons...). I found some methods online, and apparently...

10 August 2020 4,738 5 View

Latent Class/Profile Analysis AND prediction of binary outcome variable?

Hey everyone, I am trying to conduct a mixture of a latent class/ profile analysis because I have categorical and continuous outcome variables. (1) how can I combine these two? I would also...

26 March 2020 354 2 View

DdRAD final library shows low concentration at size selected peak. Anyone have any idea what could have gone wrong?

I sent off my first ddRAD library for sequencing and as QC it was first run through the bioanalyzer. I size selected with a pippin prep between 375-475 bp and the results of the bionalazyer show a...

12 January 2020 9,403 1 View

Special issue on social farming. Would do you like to contribute?

Dear all, I am Valentina Cattivelli and I am a guest editor at Sustainability (IF 2.5). With this, I would like to invite you to submit a paper within a special issue "Social farming for Social...

10 November 2019 5,392 13 View

Where to find good description of a cognitive and language development test standardization?

Hello everyone, I am new in the area of Developmental Psychology and I am looking for a good description of the standardization process of a test for cognitive and/or language development. For...

28 October 2019 2,346 4 View

How to separate human from bacterial RNA?

I extracted RNA from human saliva samples but the amount of bacterial (basically not human) RNA is very high: despite the high RNA concentration (Nanodrop quantification), after real-time PCR...

22 July 2018 4,414 3 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

How to make a time series imputation benchmark ?

Hello ! In a work, I have to benchmark different algorithms to fill in missing values in time series. I insist on the fact that this is imputation and not forecasting. In my case, I have access...

24 February 2021 7,470 7 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Can you perform the Murexide test on samples fixed in Formalin?

I have a fixed sample and would like to use the murexide reaction to test for uric acid composition. I no longer have fresh samples

22 February 2021 1,328 1 View

Monte Carlo simulation?

Hi, i am conducting a Monte Carlo simulation for my new distribution but the MSE values are increasing while the sample size increase, does any experience this issue before and what should i do...

22 February 2021 3,680 4 View

How would I use a glycerol fixation to fix whole brain Drosophila tissue?

I need to take IF images with DAPI on a confocal but I need to use a glycerol fixation. Has anyone done this method before, if so how was it done? Thank you.

21 February 2021 3,081 3 View

Is there any general protocol for immunohistology staining?

I have some fixed slides of tissue and need to do some staining, but I am not sure where to start. There are many protocols online. Any suggestions?

17 February 2021 3,062 4 View

How can a reference point be fixed to an attachment point in Abaqus?

I am currently trying to copy an assembly feature, that is present in an old version of the model I am using, onto a new model. The feature is a reference point that is fixed to an attachment...

16 February 2021 8,872 2 View

What test should I use?

15 February 2021 911 5 View