AAV9 virus purification

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Yixiong Chen Popular answer

Hi Manu，ultracentrifugation maybe the best way to purify various AAV, including AAV9.

You could find more information on this website:

www.genemedi.net/i/aav-packaging

Ali Anvar

this might be helping you:

https://www.addgene.org/protocols/aav-purification-iodixanol-gradient-ultracentrifugation/

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3609617/

Manu Prasad

thanks

Urh Černigoj

Quite late, but check the following:

http://www.biaseparations.com/applications/viruses-vlps/product/832-chromatographic-separation-of-full-and-empty-adeno-associated-virus-aav-particles-on-cim-monoliths

It was done for AAV8, but it works for AAv9 as well.

Yixiong Chen