in electrochemical sensing applications, there is usually a successive addition of droplets of a particular solution, let's say glucose solution with Concentration1= XmM, with Volume1=50uL, in another solution, lets say PBS with Volume2=100mL, and glucose concentration2=0mM (C2).
Now, if we're interested to investigate the output signal of the sensor for 1mM of the glucose, should the droplet concentration of glucose(C1) be 1mM or according to final Volume(C2=100mL), the droplet concentration of glucose should be 100mM,which will be diluted to 1mM in the final solution where the sensor is located?
According to the following equation:
C1V1=C2V2
Of course you need to add a small volume V1 of the C1 concentrated glucose solution to obtain the desired C2 glucose concentration in the V2 test solution, according to C1V1=C2V2
Thank you Eduardo. Jus to make sure I got your answer correctly. what you're suggesting is that in the problem above, the droplet concentration of glucose (C1) should be 100mM not 1mM. Is that correct?
Yes. But be careful with dilution factors. Your first addition will be only 1% of the total volume so the dilution factor is nearly neligible, but after some additions you'll need to account for it.
If you are attempting electroanalytical experiments, the volumes you are managing seem too large. Usually, electroanalytical cells contains around 10 mL solution, in this case you will need to add 100 microlitres of the concentrated solution to get the final concentration (ignoring the dilution factor).
Thank you again Eduardo. I'm in the same range that you've suggested.; And the unrealistic numbers used in the problem are only for the example purpos.
I also appreciate the explanation about the dilution factor, which is very helpful.
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