A researcher has mouse tissue expressing GFP. Can I FFPE this tissue while maintaining the GFP signal?

01 January 2019 1 1K Report

Does the processing of tissue through alcohols, xylene, and paraffin remove the fluorescent properties of endogenous transfected GFP?

Adam B Shapiro

This paper seems relevant to your question:

Chapter Green Fluorescent Protein as a Tracer in Chimeric Tissues

Also, this question has been discussed before on RG, such as here:

https://www.researchgate.net/post/What_is_the_best_fixation_for_fluorescence_microscopy_of_GFP_tagged_proteins

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