Does the processing of tissue through alcohols, xylene, and paraffin remove the fluorescent properties of endogenous transfected GFP?
This paper seems relevant to your question:
Chapter Green Fluorescent Protein as a Tracer in Chimeric Tissues
Also, this question has been discussed before on RG, such as here:
https://www.researchgate.net/post/What_is_the_best_fixation_for_fluorescence_microscopy_of_GFP_tagged_proteins
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