Hello!
I work with mouse islets and I want to detect phospho-Insulin Receptor by western blot but all the antibodies I have been testing are horrible... even with amounts of protein around 45ug...
Could anybody help me to find a good antibody? I use isolated fresh islets, i cannot perfom IP because I can't get this amount of sample...
Thank you!!!!
B
Maurice Shen
Hello Berta,
You can try searching on BenchSci (www.benchsci.com) for IR and filter for "phospho" "WB" and "Mouse".
I found several antibodies that have been published under these conditions. Perhaps you can check them out and see if there's any you haven't tried.
I hope this helps!
Maurice
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