CHO-Lec1 cells have a defective N-acetylglucosaminyltransferase gene or it completely lacks it. From the original papers it seems like they have a reduced enzyme activity due to mutations. But in some places I also came across statement that they lack the enzyme (N acetylglucosaminyltransferase )
Stanley P, et al. Selection and characterization of eight phenotypically distinct lines of lectin-resistant Chinese hamster ovary cells. Cell 6: 121-128, 1975. PubMed: 1182798
Stanley P, et al. Chinese hamster ovary cells selected for resistance to the cytotoxicity of phytohemagglutinin are deficient in a UDP-N-acetylglucosamine-- glycoprotein N-acetylglucosaminyltransferase activity. Proc. Natl. Acad. Sci. USA 72: 3323-3327, 1975. PubMed: 1059116
Hi Nilima,
It is not a complete KO, they only have a point mutation in the gene. The details are available here: http://www.ncbi.nlm.nih.gov/nuccore/U65792
"Glycosylation defect in Lec1 Chinese hamster ovary mutant is due to
a point mutation in N-acetylglucosaminyltransferase I gene"
Best. Norbert
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