I saw in another debate that I can't use that size for these cells, has anyone used it?
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?
27 July 2024 648 3 View
When I stained the presynaptic sites of NMJs in the mouse lumbrical muscle, I observed significant background in my IFI. I fixed them with 0.5% formaldehyde overnight at room temperature....
16 June 2024 3,428 0 View
Hello everyone I have a model of an area of Lisbon designed on Envi-met but the top of the model is close to the top of the buildings. When I try to add cells to the top of the model the program...
10 June 2024 3,556 0 View
Hi, I am looking for some low molecular weight organic compound/dye that absorbs light above 600 nm up to 750 nm with a decent absorptivity, that is reactive to amines or has a carboxilic acid...
22 May 2024 2,803 5 View
How do you think technology can help hospitalized children?
10 May 2024 6,508 2 View
One more opportunity of work on the science area. Title- Scientific Reviewer Duration – 12+months (extension) Location – Bethesda, MD Work Details: Complete the administrative review of grant...
08 May 2024 5,344 3 View
For factorial designs I usually see the independent variables being tested in levels (e.g., high/low). In my experiment, though, the effects of two different independent variables can only be...
05 May 2024 4,154 1 View
What requirements (or qualities) are most important to be a good university professor?
22 April 2024 3,009 5 View
Dear ResearchGate community, I received the contact of a recruiter in my email regarding another vacancy in USA. Title: Bioinformatics Training Program Manager Duration: 12 months Location:...
18 April 2024 1,544 0 View
Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...
23 July 2024 6,783 1 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...
18 July 2024 4,183 2 View
Existen diversos conflictos y problematicas sociales en el mundo que han causado migraciones. Por ejemplo, la migración venezolana y la reciente migración debido a la guerra entre ucrania y rusia....
16 June 2024 6,989 1 View
Asper discussion, First time my clonning was failed becuase the first 45 nucleotide was not cloned in pet30 a vector then I choosed the different clone of 1:1 ration fraction, I got the size of...
13 June 2024 7,564 7 View
I am working with chitosan/dextran sulphate nanoparticles loaded with rifampicin, which is fluorescent. Dsl analysis are bad and I saw that is necessary use a narrow band filter. How can I select...
05 June 2024 1,324 3 View
Which of the staining method (crystal violet/ hematoxylin) is preferred to stain the trans well migration assay membrane and why?
04 June 2024 2,766 0 View
understanding migration and population structure
31 May 2024 2,020 0 View
I try to build a three-phase wireless power transmission model and saw this module in an article.
27 May 2024 3,471 2 View
I heard this from someone: When students were assigned to defend a stance opposite to their own held view in a debate, their personally held views were permanently altered, closer to center from...
20 May 2024 4,387 1 View