I used to use refrigirated 10.000 centrifuge for DNA extraction of Filamentous Fungi
However sometimes there will be challenges and you should take the most advantage from it
The chalenge dear Audience is ...
Would it be possible to use a 6000 rpm centrifuge to extract that DNA
The protocl is here but atually it s meant for phases separation using HiGH spead constant
Do you thinkk it will work ? do you have any Protocol that we can use at this stage ?
Wish Us Good luck
Hello, all depends from relative centrifugal fore RCF. If no differences of little differences between both RCF you can use.
So If you have both centrifuge calculate RCF for both and you will get your answer.
How to calculate RCF, here the link
https://www.hettweb.com/mobile-app
Hope this info will help you!
Hello, all depends from relative centrifugal fore RCF. If no differences of little differences between both RCF you can use.
So If you have both centrifuge calculate RCF for both and you will get your answer.
How to calculate RCF, here the link
https://www.hettweb.com/mobile-app
Hope this info will help you!
Hi dear Wafaa Mokhtari
I'm not completely sure about this one but I have personally never precipitated DNA at such low speeds.
But in all honesty I would be wary and have never spun DNA at such low speeds.
Maybe you can increase the time up to 30 minutes in low speed as will cooling the rotor at 4 C but it's better to use high speed.
Therefore I would be interested myself to hear from people who have had personal experience of low speed centrifugation in order to precipitate DNA.
Good luck
I recommend to you express the centrifuge effect in terms of “ng”: the gravity times “n” of centrifuge centripetal acceleration “ac”. Where,
ac=((2piN/60)^2)r and n=ac/g
"pi" is pi: 3.14159, I do not know how to write greek letters here. “N” are the rpm and “r” the centrifuge radius. Thus you ,may express the centrifuge as 1000g, 2000g or any. It is more representative than rpm.
From experience anything less that 10000 rpm is difficult for complete DNA extraction. That is my opinion
Hi Wafaa
This reference is useful for you
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=13&cad=rja&uact=8&ved=2ahUKEwiEtIvEut3fAhXHKewKHQhLBM8QFjAMegQIBRAC&url=http%3A%2F%2Facta-arhiv.chem-soc.si%2F54%2F54-4-893.pdf&usg=AOvVaw0D7ivXPHCQMg2h2va3SVfa
Ref.2
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=15&cad=rja&uact=8&ved=2ahUKEwiEtIvEut3fAhXHKewKHQhLBM8QFjAOegQIARAC&url=https%3A%2F%2Forbi.uliege.be%2Fjspui%2Fbitstream%2F2268%2F3983%2F2%2Fnmorin_manuscript_revised1.doc&usg=AOvVaw0BtlgPqC5TZ57gecuPiJ9n
Ref.3
https://www.google.com/url?sa=t&rct=j&q=&esrc=s&source=web&cd=16&cad=rja&uact=8&ved=2ahUKEwiEtIvEut3fAhXHKewKHQhLBM8QFjAPegQIAhAC&url=http%3A%2F%2Facademicjournals.org%2Fjournal%2FAJB%2Farticle-full-text-pdf%2F9D133EF33619&usg=AOvVaw0JW8UuiDmPgXMpoZnJe5Z2
RPM is depending on method that your are going to apply for isolation of fungal DNA.
If you are using CTAB method it requires 12-13000rpm, or if following any kit instructions, it may vary ( 8000-14000rpm)
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