I am making a 1 mg/ml solution of DAB (Sigma Aldrich). I have followed most of the procedure: diluting 10 mg of DAB powder with 10 ml of deionized water and adding several drops of 0.5 M HCl.
However, after almost 1 hour of stirring with a magnetic stirrer, the black powder is not dissolved well and forms a dust cloud in the solution.
Is this problem due to the solution-making procedure or the DAB powder itself? How can I identify broken and unusable DAB powder?
Hi Raga,
Yes, this is common. I would suggest you to try 50 mM Tris-acetate buffer or PBS (pH-3- 3.5) instead of water. You can also make 0.5mg/ml stock it will also work well. After 4-5 hrs on the rocker or stirrer, filter it through a 0.22-μm filter to get a clear solution. Make sure that you stain your control and treated samples with the same lot of DAB stain. All the best!
-
Prafull
See also an earlier discussion on this topic: https://www.researchgate.net/post/DAB-33-diaminobenzidine-will-not-dissolve
- Badges
- Science topic
- Similar topics
- Agriculture
- Horticulture
- Seeds