Im making liposomes with microfluidics, with a chip and two syringe pumps (no controls and automatization). Im concerned that im getting a 3 phase product: unencapsulated oil at the top, unencapsulated aqueous solution at the bottom and a intermediate phase charge with liposomes at the middle. With the flows that I use I get them almost in equal proportions.
How could i correct this, to obtain more volume of the phase with liposomes? And become more efficient.
Why im only encapsulating a fraction?
This could be solved using a more advance system with automatic controls and pressure system?
Microfluidics method is somehow an old headache in liposomology particularly for the encapsulation of lipophilic compounds.
doi: 10.1016/S0076-6879(09)65007-2
Try other methods of liposome preparation.
M. R. Mozafari Thank you very much for your advice. Yes, im already noticing it, but for this investigation theres no moving backwards. So I have to be creative to try to solve little things.
Why three phases?
If you dissolve the encapsulated substance in one of the phases, you reduce the number of variable parameters
But in the end, I also agree with Professor Mozaffari's opinion.
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