2D10 anti-human CD80 target epitope?

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Which questionnaire/scale should I use to "measure the effects" of socioemotional development influenced by an intervention in a highschool?

Requirements for the questionnaire/scale, it shoud: "measure" socioemotional learning (see definition of SEL here: https://casel.org/overview-sel/ ); possibly fit a pre-, mid- and post test...

20 October 2020 532 3 View

Datas about silver nanoparticles biocides?

Hi everybody: i am looking for recent datas about the amount of registered biocides containing silver nanoparticles. The most recent data i found is in this paper from Nowack et al. (2011) which...

23 April 2020 353 1 View

Analysis reproduction data in chronic toxicity tests with Ceriodaphnia dubia?

Hi everybody. I am facing an issue about reproduction data analysis in chronic toxicity test with C.dubia. US EPA Method 1002.0 (...

13 March 2020 1,932 4 View

NEED HELP PLEASE: How much AgNO3 salt i need to have a stock solution with the concentration of 5 g/L of Ag?

I have to do few toxicity tests in which silver is involved. To do that i have to prepare a stock solution using silver nitrate (AgNO3) salt. The concentration of exposure in the tests is referred...

18 January 2020 8,179 2 View

Are you aware of any study about consequences of landslide restoration/stabilization for animals?

Dear all, I have been searching through the literature for examples of possible impacts on biological communities of interventions targeted at restoring / stabilizing landslides. I found just a...

16 August 2019 6,793 6 View

Abaqus - Stress discontinuity after seismic analysis of earth dam?

Hello, I'm a student and I'm doing my master thesis work on modeling the seismic response of earth dams with abaqus, more specifically by doing dynamic non linear analysis. I did the analysis...

29 November 2018 3,222 4 View

Mantis eating horsehair worms?

Hy there, I was searching materials for my MsC thesis about horsehair worms, when I found...

13 December 2017 1,153 4 View

Heterogeneous nucleation of hydroxide on the nanoparticle surface

Hi, I am a master's student, I am trying to coat the Silica nanoparticles with LiOH. To make possible the LiOH precipitation on the Silica surface I chose to exploit the non-ideality of an...

06 July 2017 1,688 1 View

How to increase the adsorprion of Lithium Hydroxide on the Silica surface?

Hi, I am working with an alcohol solution saturated with LiOH and I need to make precipitate LiOH on the Silica surface, in particular I would get an uniform coating of the nanoparticles that I...

04 July 2017 4,375 2 View

Problems with RNA extraction from microvescicles..anyone can help me?

Hi everyone! do you have any piece of advice about RNA extraction from microvescicles? I usually recover MVs from human monocytes supernatant. I tried different protocols, like trizol and columns...

15 January 2017 7,926 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View