Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

How to Implement Elastoplasticity in a PCL model in COMSOL Multiphysics?

I am using COMSOL Multiphysics 5.5. I am trying to simulate the tensile test of PCL scaffolds. However, regardless of the strain, I have the elastic region all the time which is not true when it...

23 February 2021 4,050 1 View

Can you help me with a free /no cost publication journal in oncology Nursing?

Wish to publish an article but in a cost-free journal. Need the assistance of researchers on good indexed journals free of predatory. Thank you

23 September 2020 5,372 3 View

How do you differentiate your different populations in your FASTA file in R ?

I need to identify my populations from my FASTA file in R so that R can then run all my stats. However, I do not know how to tell R how to group my data into 4 populations! This is the warning...

09 September 2020 8,875 2 View

Can anyone please post a link to a reliable rat cDNA primer database?

I am looking for a source of cDNA primers, rat genome, real time PCR. Previously I used rtprimers bd, but is seems to be out of service for a while now.

01 September 2020 5,132 3 View

How to generat a ASCII format data in GIS?

Dear you all: Recently i met with such a problem: i tryed to generate a ascii format buffer in gis, but i failed because it's headers are different from the research data. Failed result are shown...

11 August 2020 5,040 3 View

Do 3 light sources with same intensities, but different wavelengths(blue,green, red) provide same amount of energy for photosynthesis?

As it is not directly my field, I would like to ask for help in terms of the correlation between light intensity, wavelength, frequency and amplitude. Let's say, I have a radiophotometer which...

29 April 2020 9,141 2 View

Constitutive expression of a gene in E. coli - In search of plasmid?

Dear All, I am currently working on a project based around an antibiotic resistance enzyme and I got some really interesting results. I am looking to confirm whether the enzyme under...

19 June 2019 7,700 4 View

How does one identify and extract statoliths from cubozoans?

Specifically, I am looking at the species Alatina alata. I am having trouble both finding the statoliths and also extracting them for further analysis. I have been freezing specimen before I am...

24 April 2019 7,943 2 View

Which test to use in SPSS?

So i have a study with both males and females, they will both be shown a negative image and their brain reaction recorded and a positive image with their brain reaction recorded what analysis in...

30 March 2019 4,571 6 View

No title

03 March 2021 5,360 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How the Ground Plane implantation is performed without damaging (and w/o varying the undoped nature) the top Si (8-10 nm) in FDSOI technology ?

It is a general practice to introduce Ground Plane in FDSOI devices under the BoX. The Ground Planes are introduced by Ion Implantation through Top Si layer and Buried Oxide. I wanted to know that...

24 February 2021 5,974 4 View

Could anyone recommend papers that focus on mixed-ethnicity Black-Caribbean/Caucasian adopted children?

Dear All, I have been looking for papers, or books, that focus on the issues surrounding adoption of mixed Black-Caribbean/Caucasian children. I am interested in literature that looks at any...

22 February 2021 8,466 2 View

Any way to separate out fluorescent channels from a composite image?

Hi there, I've been using the EVOS Auto II microscope for some time now and usually I will save the transmitted light/bright field image and the images from fluorescent channels as separate...

15 February 2021 546 3 View

Please, How can I plot a channel capacity of MIMO and SISO in matlab?

CSISO= f(frequency) : plot(f, CSISO) ? CMIMO= f(frequency) : plot (f, CMIMO) ?

13 February 2021 5,225 4 View

What/How to focus on with regard to research publication?

I'm an academician for past 10 years and love to continue with the same, a question pops-up at in mind from time to time, what to focus more on? Conference papers, Journal paper, book chapters,...

09 February 2021 1,009 5 View

The reason of PMMA channels cracking?

07 February 2021 3,663 2 View

How can I reduce run-to-run variation in TMT MS experiments?

I planned an experimental setup using 10-plex TMT with 9 conditions and 1 bridge channel to compare 5 replicates. But since I experienced some run-to-run variation, I was wondering if a better...

31 January 2021 3,405 3 View

What method should I use for time-varying signal analysis?

Hello! We have a project where participants engaged in reading, thinking and then responding to an ethical dilemma. We used the EMOTIV 14 channel headset to track brain activity. The reading,...

29 January 2021 4,182 3 View