I extracted RNA from spinal cord tissue using Trizol and following invitrogen's protocol for RNA extraction for PCR.
I had good 260/280 ratios (around 1,8-1,9), but very low 260/230 ratios (0,4-0,5).
I have problems to get new tissue and do the extraction again. Is there any protocol to purify the samples and try to obtain better 260/230 ratios?
Thanks
Andrei S. Babenka
Dear Raquel
My experience tell me that 260/230 ratio is not crucial for RNA samples. in the case of trizol it is quite popular problem. As I think it is very important to estimate RIN using Agilent Bioanalyser. If you can't, please use agarose gel (2%) to see 2 bands of rRNA (18S + 28S).
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