I have a tumor peptide in buffer (cytosolic protein) from cancer cell. For some experiment I have to make 20uM peptide solution. How I can make this whose MW I don’t know.
Your question lacks relevant details. What do you mean by a tumor peptide? Is this a purified protein or are you talking about a cell lysate from a cancer cell. Or is this a synthesized peptide? Can you size the protein on an SDS/PAGE gel? The short answer is you cannot calculate molarity if you do not know molecular weight.
Hello,
I am using these calculators from sigma
f.e. this mass molarity calculator (there is a lot more)
Hope it helps.
http://www.sigmaaldrich.com/chemistry/stockroom-reagents/learning-center/technical-library/mass-molarity-calculator.html
Your question lacks relevant details. What do you mean by a tumor peptide? Is this a purified protein or are you talking about a cell lysate from a cancer cell. Or is this a synthesized peptide? Can you size the protein on an SDS/PAGE gel? The short answer is you cannot calculate molarity if you do not know molecular weight.
Hi Pramod,
There is not enough information regarding this peptide. If you know the sequence of the peptide (and other info: modifications, disulfide bonds etc.) use the online peptide calculator (such as https://www.genscript.com/ssl-bin/peptide_mw) to estimate the molecular weight.
I am sure there are several sequence databases available online (for example Genscript as mentioned above) where you can get the mol. wt of your peptide. Alternatively, use 100Da for each amino acid (20aa peptide = ~2kDa) to calculate the amount of peptide you need to get particular conc. Use Tocris website to get the amount of peptide you need to prepare 20uM solution.
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