100% Methanol Fixation for IHC

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How can I determine if a drug binds to a ribosome using a Cryo-EM map?

Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...

19 July 2024 2,155 0 View

How to write a paper method for interview in a qualitative research ?

Hello, Currently, I am writing my master thesis proposal. I have been to provide a method paper for (question creation and interview evaluation). It's my first time planning to conduct...

27 April 2024 4,710 1 View

A question about antibodies?

I am hoping that someone can help me find an antibody for GABA, GAD 65 or GAD 67 that works well in marmoset cerebral cortex Thanks

21 February 2024 7,493 2 View

Where to Find Dataset of Requirements Gathering Scripts and User Stories Extracted from Requirements?

Hello, I am looking for a dataset of requirements gathering scripts and user stories extracted from those requirements. Anything similar would be helpful. Thank you.

01 February 2024 4,604 0 View

Please fill my research questionnaire ?

Hello , I hope this message finds you well. I'm a final-year civil engineering student working on a dissertation about the sustainability of ancient Egyptian construction techniques and their...

14 November 2023 987 1 View

How can I confirm the western blot band is identical with my target protein?

I used some polyclonal western blot antibody, and that datasheet size is 45-50kDa I also get the band in expected size range, but there's multiple other band(at least 3 bands in other size), and...

25 July 2023 5,349 5 View

[PROCESS Macro for SPSS] What are the Reference and Counterfactual Values of X?

Hi! I am trying to run a model 4 mediation with the xmint option (what was once model 74) but PROCESS produces the following error: "A reference value of X is required for this model." I've...

24 May 2023 6,965 2 View

Can I get some cell seeding/plating help?

Hello, I would some help with seeding/plating cells. I counted cells using the hemocytometer. I got 1,025,000 cells per mL. I need to seed 250 cells per well for 36 wells and each well has...

26 March 2023 2,481 3 View

Tritox-100 permeabilzation for immunocytochemistry?

what is the recomended concentration and duration to permeabilize mitochondrial membrane for ICC?

26 March 2023 1,958 9 View

Will a signal peptide still be cleaved if GFP is fused in front of it?

I am studying the subcellular localization of a protein. To see if a GFP fusion protein can be compared to the native protein, I fused GFP to the N-terminal of the protein. In my western blots and...

01 March 2023 749 0 View

Does continues partial charging/discharging do any harm to the BESS performance and degradation?

For a grid connected BESS (lets say 100 MW/200 MWh system, LFP battery), if we charge the BESS in such a way that whenever we can access power from the solar that will charge the BESS. Hence it...

01 August 2024 9,903 7 View

How to Post-fix Mice pup's brains?

I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...

31 July 2024 1,123 4 View

Can I change concentration and brand of antibodies mid-experiment?

I am doing immunohistochemistry of the c-Fos protein and I have a budget problem. For context, the experiment has been done over 3 years prior to my arrival and I have 40 brains done and 100 left....

29 July 2024 5,961 2 View

Comparison of Methanol and ethanol treatment on collagen based scaffolds?

Kindly share a research paper that explains the effect of Methanol and ethanol treatment on collagen based scaffolds. How it increases porosity and swelling. Kindly help me find a paper. Thank you

24 July 2024 3,937 0 View

I am trying to find phonon, and when i run calculations, there is error ?

how to fix this error, from phq_readin : error # 1 DFPT with the Blochl correction is not implemented I am attaching my ph.in file.

23 July 2024 4,649 2 View

Sodium azide in immunohistochemistry?

Hello everyone, A collaborator sent us his protocol in order to be able to use his immunohistochemistry kits. But one step contains PBS + 0.3% H2O2 + 0.1% sodium azide. Knowing the dangerousness...

23 July 2024 6,314 3 View

How to fix install package meta in R?

> library(meta) Error: package or namespace load failed for ‘meta’ in loadNamespace(i, c(lib.loc, .libPaths()), versionCheck = vI[[i]]): there is no package called ‘numDeriv’

18 July 2024 3,536 0 View

GIS (How can i fix when it appear in mines despite selecting WGSWGS 84 / UTM zone 38N?

I have a problem with geo-referencing a shape file. How can I fix it when it appears in mines despite selecting WGSWGS 84 / UTM zone 38N?

18 July 2024 2,696 1 View

Why in some wavelengths there are "overflows" in my Carbon dot UV-Vis Spectroscopy results(Emission segment)?

Hi everyone I have got this test in a fixed excitation and various emissions. In some wavelengths there are overflows which make the resulted analyze different from other carbon dots. Is it normal...

18 July 2024 4,244 2 View

How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

17 July 2024 7,767 3 View