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What are the reasons of fluorescence decline in plateu phase of qPCR?

Hello! I am optimizing conditions of stem-loop RT-PCR to compare the expression of miRNAs in different cell lines. Now I'm performing the reaction in one step: RT with stem-loop primer and...

17 October 2024 2,823 2 View

Alternative Methods for Electroporating DNA into NK92 Cells and Low-Cost Electroporator Recommendations?

Dear Everyone, We are currently working with NK92 cell lines, but unfortunately, we do not have access to electroporation equipment such as the Neon Transfection System or Nucleofector Device....

17 October 2024 4,109 0 View

Do you know formation age of Europe Coals?

I want to know more about age of coal formations.

16 October 2024 6,475 1 View

How i can dissolve Chitosan in HCl (aq) ?

I need prepare a dissolution of chitosan without acetic acid.. And i need prepare it around pH=5, not lower Thanks

15 October 2024 7,928 2 View

Total hardness (TH) remains relatively unchanged (no significant change), while TSS and TDS decrease during water treatment. Why?

I am doing the research for water treatment regrading photocatalysis process. In the comparison of blank (wastewater) samples with treated water samples, the following observations were made: 1)...

15 October 2024 2,911 2 View

How to improve the hydrogen storage density of polymer-based hydrogen storage materials?

From the perspective of optimizing the structure and composition of materials.

14 October 2024 6,118 2 View

Why superabsorbent resins can absorb water hundreds or even thousands of times their own weight?

Please explain the water absorption properties of the resin from the microstructure.

13 October 2024 6,086 2 View

Why can superabsorbent resin prevent water leakage and evaporation?

Please analyze from the perspective of microstructure.

13 October 2024 5,883 1 View

How to denote letters to mark significant differences in a bar chart plot?

I want to add lettering on bar chart bars. How to add?

13 October 2024 8,550 1 View

What are the main applications of foam carbon?

The foam carbon here refers to the multi-level foam carbon after foaming. I want to know what fields it can play a role in

13 October 2024 1,635 2 View

Which theoretical framework should I use to indentify challenges facing SOEs in South Africa and how to use them?

Basically how to use that theory to identify the problem? eg. Agency theory, Stewardship Theory, and Public Choice Theory.

10 October 2024 6,843 2 View

Is there a convenient method for extracting phenanthrene from soil and determining its concentration?

I'm doing an experiment on PHE degradation in soil, and one of the procedures involves measuring the amount of phenanthrene in a sample of phenanthrene-contaminated soil with a high water content,...

10 October 2024 4,244 1 View

Is angiogenesis/vascular remodeling beneficial for the improvement of the disease in clinical ischemic stroke patients?

Are there any related literature references? Thank you.

09 October 2024 609 3 View

I am studying phd in Universiti Sains Malaysia, I looking for one share me in publication paper in field instructional technology ??

Highly welcome

05 October 2024 1,766 0 View

What are the requirements for implementing green education in primary schools?

تحديد متطلبات تطبيق التعليم الأخضر في المدارس الابتدائية

02 October 2024 8,130 6 View

Can green education be applied in foreign language subjects?

أمثلة عن تطبيق التعليم الأخضر في مواد اللغات الأجنبية

02 October 2024 1,959 3 View

What are the most appropriate educational materials and curricula in which green education is applied in primary schools?

توضيح أنسب المواد والمناهج التعليمية التي يطبق فيها التعليم الأخضر في مدارس التعليم الاساسي

01 October 2024 1,600 5 View

What are the advantages of teaching using artificial intelligence in children's education?

The Advantages of teaching using artificial intelligence in children's education in schools

28 September 2024 6,268 5 View

How to detect Kras mutations and distinguish between mutation types, such as KrasG12C or KrasG12D, using biological or chemical methods?

The causes of KRAS mutation types in cancer screening typically involve genetic factors, environmental exposures, DNA replication errors, inflammation, and oxidative stress. KrasG12C mutations are...

23 September 2024 9,787 1 View

Why does my protein show very high fluorescence at low temperatures in a TSA?

I am trying to investigate the thermal stability of a protein I work with using the TSA with SYPRO Orange. However, even though my protein produces a peak at about 43 degrees, it shows very high...

16 September 2024 5,596 4 View

Activity inversely proportional to fluorescence?

Hi, I am performing an enzyme activity assay where Fluorescence is quenched upon successful conversion of substrate to product and the it is an endpoint assay. How do I calculate % activity or %...

12 September 2024 3,838 3 View

Why my primers amplify genes of other organisms?

Hello everyone. I would be really grateful if you can help me solve my problem. I have purchased Real Time PCR primers for ureC gene (glmM) of Helicobacter Pylori which the sequences were in an...

10 September 2024 186 3 View

Does anyone have a list of 19th century newspapers housed at the Archive General del Estate de Veracruz in Xalapa?

Looking for a listing of 19th century Veracruz newspapers (especially Port of Veracruz) held at the AGEV-state archive of Veracruz in Xalapa. Thank you, AGW

09 September 2024 8,657 0 View

How i can download some articles for my project and seminar exactly same detail?

omparing the mechanical and metallurgical properties of automatic MIG welding in various passes with single and dual torches on X65 grade oil and gas pipelines

06 September 2024 9,081 0 View

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