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Questions related from Ziming Chen
Dear all, Recently we were doing confocal live cell imaging on Mitotracker staining to a osteocyte cell line (see attached pic.). However, we found some black dots in background (black dots are...
07 February 2023 1,288 1 View
Dear all, Recently I plan to treat cells with a transmembrane protein (32 kda) and I hope cells can uptake it. I read some paper about cell uptaking protein and seems package it in liposomes or...
08 January 2023 8,801 1 View
Dear all, I am quite new in cytoskeleton area. Recently I am finding a gene coding F-actin. But then I found there is not a gene called F-actin but Acta1. After searching some information, I...
17 April 2021 5,805 2 View
Dear all, Recently I plan to do some transient transfection for overexpression experiment. As the plasmid of my target gene on Addgene is hard to use (need some subclone) and our group has not too...
27 November 2020 3,293 4 View
Dear all, Recently I am using Lipofectamine 3000 to transfect plasmid into my primary cells. However, the efficiency of the cells is unsatisfactory. Then I find this product, PLUS reagent. As it...
11 November 2020 6,532 0 View
Dear all, Recently I plan to test a protein for its potential chemoattractant property for macrophages chemotaxis. And I plan to test on both mouse and human cell. I realized some used RAW264.7...
10 August 2020 7,415 13 View
Dear all, Recently I plan to observe mitophagy by mt-Keima. Many article used it by a live cell imaging. Is there anyone know if I can observe mt-keima after fixation. Thank you. Best Regards.
16 July 2020 2,184 1 View
Dear all, I am looking for the primer of mitochondrial 16s rrna and 18s rrna in human and mice. I just find the primer for h 16s rrna and h 18s rrna from a paper. However, the blast results are...
14 July 2020 5,907 3 View
