Yi Zhou

4 Questions 4 Answers 0 Followers

Questions related from Yi Zhou

The resolution verification of an optical microscopy system?

Dear all, When experimentally demonstrating the resolution of an optical microscope by grating samples, should we use the lindewidth or periodicity of the grating to mark the resolution? For...

04 March 2025 8,100 1 View

Why the lateral resolution of OLS5100 (Olympus) can be down to 120 nm?

The laser confocal system of OLS5100 (Olympus) can reach a lateral resolution of 120 nm, that is far beyond the diffraction limitation. Though the numerical aperture of its system is 0.95, the...

16 May 2024 1,510 3 View

Rayleigh criterion: Saddle-to-peak ratio is 81.1% or 73.5% when imaging gratings?

Hi all, Optical microscopy resolution limit is explained by Rayleigh criterion (widely applied). We have noticed that some papers show that Saddle-to-peak ratio should be 81.1% if the structure...

09 July 2022 7,302 0 View

The reasons causing artifacts around the microscopy image?

Hey, We are developing a general transmission microscopy system under 405 nm illumination. A high NA objective with 150 X magnification is used. From the image we can see there are several halo...

23 December 2021 5,201 5 View