Ubaid Rasool

9 Questions 11 Answers 0 Followers

Questions related from Ubaid Rasool

Is it appropriate to use reverse sequencing data for BLAST analysis?

I have got the reverse sequencing data of the organism and I want to go for BLAST search. If I'm using the reverse sequencing data for BLAST analysis, will it provide the accurate results?

24 July 2018 6,743 2 View

Can nanoparticles have two opposite effects?

I have synthesized iron oxide nanoparticles and evaluated their antibacterial and anticancer activity. The results which I got were opposite for both these experiments. Iron oxide nanoparticles...

21 April 2018 1,893 4 View

Can there be a difference in the activities of nanoparticles in liquid and solid media?

Is it possible that nanoparticles can have varied antifungal properties in liquid and solid media? Is the interaction more in the liquid media and hence the greater activity?

07 March 2018 4,787 5 View

What to do when reverse sequencing results show more resemblance to some organism than forward sequencing resuts in BLAST ?

I did the forward and reverse sequencing of my sample and after matching the sequences in BLAST, the reverse sequences matched 95% with some uncultured organisms, but the forward sequences matched...

15 February 2018 1,821 4 View

What to do when sequencing results do not properly matching with any organism in ncbi ?

I did the PCR of the isolated DNA from actinomycetes and later i carried out the sequencing of the isolated actinomycetes. I did the BLAST analysis later but it's matching with different...

05 February 2018 7,788 3 View

What's the mechanism of action of nanoparticles on Candida albicans?

How do nanoparticles act on Candida albicans? Like for gram positive bacteria, the nanoparticles penetrate the cell wall and kill the bacterial cells. Is the same mechanism followed by...

11 June 2017 182 3 View

How to determine MIC and MIC-50 using 96 micro well plate ?

I have performed serial dilutions of my sample to get different concentrations and inoculated the bacteria using a 96 micro well plate. I took the OD600 using multimode reader. Now, Shall i...

17 May 2017 9,490 2 View

How to get an information about the crystalline structure of nanoparticles from XRD data?

I have synthesized copper nanoparticles and characterized them through XRD. I need to interpret the XRD data so that I can get an information about the structure. Is there any online software that...

24 April 2017 4,460 3 View

How to determine the concentration of copper nanoparticles in a solution ?

I have synthesized copper nanoparticles from bacteria. Now I have a solution containing copper nanoparticles. I have already gone through the spectrophotometric analysis of these nanoparticles....

25 February 2016 5,137 3 View