Tuba Sena Ogurlu

6 Questions 15 Answers 0 Followers

Questions related from Tuba Sena Ogurlu

Although the ladder transfer is okay, I can not see my proteins on the membrane, what is the reason ?

Hello, I have been doing western-blot experiments for 8 months or something (Invitrogen B1000 Mini Blot Module). I used to get good results until 2 weeks ago. Then something happened, I could not...

30 August 2023 6,980 3 View

How to compare means of groups separated by facet_grid function ?

Hello everyone, I am not good at R so I am trying to find solutions for my problems through the internet. I have been stuck on a problem. I couldn't find a way to compare the means of groups...

10 June 2023 8,727 6 View

Is there any package in R to find mean ranks ?

Hello, I have data for which I have to use the Mann-Whitney U test. Apparently, It would be better to show mean ranks rather than mean or median of the samples. I only use R for data...

30 April 2023 8,357 2 View

Can I run a qPCR experiment with gradient temperature or should my targets be amplified at single temperature ?

Hello everyone, I am running a qPCR assay. I chose gradient temperature option for each of my primer to get the best conditions the amplification happens (without heterodimers- NA in negative...

08 November 2022 7,864 6 View

How to normalize one data according to another data ?

EDIT: Please see below for the edited version of this question first (02.04.22) Hi, I am searching for a reliable normalization method. I have two chip-seq datas to be compared with t-test but...

01 February 2022 4,309 12 View

Should I remove zero rpkm values when I examine a ChIP-seq data ?

I have two different ChIP-seq data for different proteins, I have aligned them to some fragments in the DNA. Some of these fragments get zero read count for one of them or for both. To be able to...

01 February 2022 4,989 3 View