Tuba Sena Ogurlu

3 Questions 7 Answers 0 Followers

Questions related from Tuba Sena Ogurlu

Can I run a qPCR experiment with gradient temperature or should my targets be amplified at single temperature ?

Hello everyone, I am running a qPCR assay. I chose gradient temperature option for each of my primer to get the best conditions the amplification happens (without heterodimers- NA in negative...

08 November 2022 7,855 6 View

How to normalize one data according to another data ?

EDIT: Please see below for the edited version of this question first (02.04.22) Hi, I am searching for a reliable normalization method. I have two chip-seq datas to be compared with t-test but...

01 February 2022 4,291 12 View

Should I remove zero rpkm values when I examine a ChIP-seq data ?

I have two different ChIP-seq data for different proteins, I have aligned them to some fragments in the DNA. Some of these fragments get zero read count for one of them or for both. To be able to...

01 February 2022 4,972 3 View