11 Questions 8 Answers 0 Followers
Questions related from Tang Zhi Zhuang
Hi all, I am writing manuscript for cloning research, I want to ask if there's any software that allow us to generate nucleotide and amino acid sequence in one same figure, I have been doing using...
16 January 2020 6,448 3 View
Hi all, Currently working on in-gel digestion for peptide identification, other than using ziptip or centrifugal unit to desalt sample, I heard using TCEP also works well to desalt. I wish to...
30 April 2019 6,921 2 View
Hi all, there is a question i am curious, my friend currently expressing recombinant protein using pQE30 vector, is it possible that protein able to be induced without addition of IPTG? As I know,...
03 January 2019 3,519 2 View
Hi, I am currently studying a paper related with plant immunity (PTI & ETI), I noticed other papers would used BiFC as a tool to study protein interactions rather than FRET, any particular...
28 February 2017 1,748 2 View
Hi all, i am currently using rosetta competent cell to expressing my target protein, I incubated to OD~0.4, using IPTG induction in 25C overnight, however the next day OD only ~0.5, it did not...
29 November 2016 3,430 2 View
Hi all, I am currently expressing a membrane protein on eukaryotic cell line, the morphology observed have similarities with other eukaryotic cell line expressing same protein, but is there any...
05 October 2016 7,921 3 View
Hi all, I successfully expressed receptor protein in e.coli expression system, and its in soluble form however, when i proceed to purify it, there is no binding to the column(current using...
14 September 2016 8,813 12 View
Hi all, I am currently trying to express a type of cell-surface receptor(full length) using E.coli bacteria, however, i only can get small amount of protein expressed after ran SDS-PAGE gel, i...
28 August 2016 8,446 6 View
Hi all, I am currently ligating 1.3 kb gene to pET29 vector, however, i cant get any bands after colony PCR using T7/T7t primers. I gotten a clear one band in RE digestion for clean cut vector and...
01 August 2016 9,820 4 View
What is differences between expression of haemagglutinin or neuraminidase surface antigenic in influenza viruses? Expressing recombinant HA have better antigenicity than neuraminidase?
27 May 2016 3,267 2 View
Hi, i wanna ask if there any vectors can be use to ligate 1.4 kb fragment other than pgem T-easy or T vector?
09 May 2016 2,983 5 View
