Sueden de Souza

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Questions related from Sueden de Souza

How to Adjust MTT Readings for Blank & Reference Wavelength?

Hi everyone. I'm using MTT for cytotoxicity experiments with Vero cells. My question is about the calculations. I measured my samples at two wavelengths: - 570 nm - Reference: 630 nm Please let...

14 April 2022 3,683 3 View

Can C. albicans culture change its phenotype?

Hi everyone! I'm working with cultures of two C. albicans strains and one of them (ATCC 10231), which was a gift from a partner lab from about 6 years ago has lost its ability to form biofilm. I...

21 October 2021 3,842 0 View

How to change the magnification of a SEM image?

Hello! I have a few images of fungal biofilms acquired through SEM but they all have different magnifications (i.e. 160x, 140x, 110x and 100x). Does anyone know a way to change the magnification...

10 June 2021 2,613 8 View

What cell density range can MTT detect?

Hello! I've used MTT assay to assess the viability of Canada biofilms after a treatment. I had about 90% decrease in cell viability from MTT assay, but I don't get the same expressive result when...

09 February 2021 8,314 4 View

What composition of culture medium Yeast Nitrogen Base is best for Candida biofilm formation?

Hello! I am trying to grow Candida albicans biofilms in the lab. So far, I've been using Sabouraud Dextrose Broth, but many articles describe Yeast Nitrogen Base - YNB for Candida biofilm...

04 September 2020 8,392 4 View