Stav Kozlovski

4 Questions 3 Answers 0 Followers

Questions related from Stav Kozlovski

FACS protocol for staining of CD41 on lung platelets?

Hi,I would like to stain for the surface expression of CD41 on lung platelets. For FACS staining of the lung I usually perfuse and extract the lung, digest the tissue with Collagenase 4 and...

28 November 2016 9,827 1 View

What are TCR transgenic mice models for CD8+ effector cells?

Hi! We are currently using the ovalbumin-specific TCR transgenic OT-1 mouse model. I activate the CD8+ cells by exposing the splenocytes to the siinfekl peptide and after 72hr CD8+ cells have...

15 October 2015 774 2 View

What is the difference between 293F (freestyle) and 293T for transfection?

Hi, I would like to transfect 293 cells, what is the difference between using 293T and 293F? Thanks!

18 August 2015 3,456 4 View

What cell line are B16a melanoma cells derived from?

Hi! I've been trying to find out from what cell line are the B16a (amelanotic melanoma) cells? Are they derived from B16 - F0 or maybe from B16-F10? any insight would be greatly appreciated Thanks!

09 August 2015 7,906 3 View