Hi, I am trying to subject a biodegradable polymer, Polycaprolactone (PCL, MW: 50,000 kDa) to enzymatic degradation for a period of 3 weeks and assess the extent of degradation by tracking the change in parameters like: crystallinity, glass transition temperature (Tg) and melting point (Tm) using differential calorimetry.
I am trying to come up with a protocol but there seems to be so much variance with respect to heating/cooling rate. I have tried three different rates 5, 10 and 20 degrees/min and it seems to have a profound effect on the thermal property peaks. Hence, I wanted to get some suggestions regarding the suitable heating/cooling rate for performing DSC.
Also, should I use hermetically sealed or non-sealed Aluminum pans for packing my powdered samples. My working temperature range is -80 to 80 degree Celsius.
I really appreciate your help. Thanks!
Normally, the standard protocol to determine these parameters is:
-1st run
Ramp 10°C/min. to 80°C
Isothermal 5 min.
Ramp 10°C/min to -80°C
Isothermal 5 min.
-2nd run
Ramp 10°C/min. to 80°C
Isothermal 5 min.
Ramp 100°C/min to -80°C
Isothermal 5 min.
-3rd run
Ramp 10°C/min. to 80°C
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