Srilatha Badaboina

9 Questions 21 Answers 0 Followers

Questions related from Srilatha Badaboina

Query related to JC-1 mechanism

JC-1 dye is well known for using mitochondrial membrane potential disruption detection during apoptosis. JC-1 entry is from the cell membrane to the cytosol to the mitochondria and forms...

11 November 2012 5,017 15 View

Is it possible to use Alamar blue to test cell cytotoxicity at different time points with the same sample ?

So far we've been using MTT for cell cytotoxicity. We purchased alamar blue from invitrogen newly: how can we use same 96 well plate reading after 1 day,2 day,3 days? As we know alamar blue is...

09 September 2012 9,079 3 View

Is it possible to use Alamar blue to test cell cytotoxicity at different time points with same sample ?

All these days we were using MTT for cell cytotoxicity. we purchased alamar blue from invitrogen newly, how can we use same 96 well plate reading after 1 day,2 day,3 days. Please share your...

09 September 2012 7,576 5 View

Does an increase in caspase activity mean apoptosis should increase?

PI3k inhibitor(LY294002) and our test compound showed a 7 fold increase in caspase 3/7 activity . We further tested apoptosis by annexin V FITC+PI by flow cytometry. We found that in the PI3k...

08 August 2012 4,174 14 View

Sample preparation in PCR machine for SDS-PAGE?

I generally aliquote cell lysate sample in PCR tubes after protein estimation. Can I add loading dye to a sample in PCR tubes and heat the samples at 95C for 5min in PCR machine? Is it ok doing...

06 June 2012 9,732 2 View

My HepG2 cell line is growing very slowly, why?

I am trying to grow hepatic cell lines like HepG2, HUH-7 and ,Hep-3b for anticancer work. I am growing all in RPMI-1640 + 10%FBS + 1X Ab/AM. HUH-7, and hep-3b are growing very nicely but HepG2...

04 April 2012 4,386 10 View

how to calculate IC50 using sigma plot software.

I do MTT assay to see the cell viability upon drug treatments.we have sigma plot.10 software programme.could anyone tell how to calculate IC-50 using that.

02 February 2012 9,358 5 View

Cleaved caspases detection

I would like to detect cleaved caspases (17,19 kd) so Is 15% SDS PAGE ok for this? running SDS PAGE 25mA for 1hr and western transfer conditions i use 100V for 1hr. and any suggestions during...

12 December 2011 3,713 1 View

Solvent for partially purified product?????????

We have some plant product partially purified powdered form,i need to check anticancer activitiy of that.iam dissolving product in Methanol and further dissolving in growth medium for MTT...

12 December 2011 6,550 3 View