Shahid Chaudhary

4 Questions 16 Answers 0 Followers

Questions related from Shahid Chaudhary

Can someone suggest a gRNA cloning strategy?

I am naive to this CRISPR/Cas9 system for gene editing. As a part of my research project, I want to establish this protocol in my lab to knockout the E6 and E7 oncogene of HPV16 and HPV18 virus. I...

12 December 2014 3,557 7 View

Blunt PCR product cloning?

I am trying to clone my blunt PCR product amplified by Deep Vent polymerase into the TOPO vector. The PCR product is of size approximately 900 bp. The product concentration is 90.1 ng/ul as...

01 January 2014 5,502 24 View

Can anyone help with adding linkers to PCR amplified product?

I a trying to attach Linkers to the FAM3A gene which I have amplified from the genomic DNA of the patient. PCR worked great for the amplification of FAM3A gene. Now I am trying to attach linkers...

12 December 2013 6,152 4 View

Site-directed mutagenesis?

I am trying to perform site-directed mutagenesis for which I have designed primers carrying the desired mutation (the primer is designed by my professor). My plasmid length is 9 Kbp. I am using...

11 November 2013 9,675 33 View