Senthilkumar Thirumurugan

3 Questions 2 Answers 0 Followers

Questions related from Senthilkumar Thirumurugan

Low Transduction Efficiency Using Retroviral Transduction with Platinum-E Cells — Any Suggestions?

Hi everyone, I’m currently performing retroviral transduction of primary T cells using viral supernatant generated from Plat-E (Platinum-E) packaging cells. I’m using Retronectin-coated plates to...

06 August 2025 3,545 1 View

At what time sofium butyrate needs to be added in pei max based transfection?

I’m doing pei max transfection in platinum E cell lines to collect retrovirus supernatant but my transduction is very low. if I add sodium butyrate as booster for enhancing transfection and...

30 July 2025 4,922 2 View

Doubts in MMP

Hi everyone, Im going to do Mitochondrial membrane targetting of breast cancer cell by using nanoparticles. I saw many papers but they didnt mention what concentration of NPs to use? can u...

01 January 1970 2,034 1 View